Purified anti-Nestin Antibody
- 产品名称：
- Purified anti-Nestin Antibody
- 产品类别：
- 抗体
- 产品编号：
- 655102
- 产品应用：
- 655102
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Homogenized Sprague-Dawley rat spinal cord.
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- Affinity purified.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The reactivity of Alexa Fluor? 647 anti-Nestin antibody was verified in FFPE embryonic mouse tissue.
- Application References
(PubMed link indicates BioLegend citation) -
- Hockfield S, et al. 1985. J. Neurosci. 5:3310. (IP)
- Friedman B, et al. 1990. J. Comp. Neurol. 295:43. (IP)
- Brook GA, et al. 1994. Exp. Neurol. 126:31. (IP)
- Lendahl U, et al. 1990. Cell 60:585. (IP)
- Product Citations
-
- Tu J et al. 2018. Stem cell research. 28:29-32 . PubMed
- RRID
- AB_2562023 (BioLegend Cat. No. 655102)
- Structure
- Mouse nestin is 1864 amino acids with a predicted molecular weight of 207 kD. The observed molecular weight is larger than 250 kD.
- Distribution
-
Cytosol
- Function
- Nestin gene encodes a member of the intermediate filament, and it is required for survival and renewal of neural progenitor cells.
- Cell Type
- Neural Stem Cells
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Antigen References
-
1. Lendahl U, et al. 1990. Cell 60:585.
2. Gilyarov AV, et al. 2008. Neurosci. Behav. Physiol. 38:165.
3. Méndez-Ferrer S, et al. 2010. Nature 466:829.
4. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
- Gene ID
- 18008 View all products for this Gene ID 25491 View all products for this Gene ID
- UniProt
- View information about Nestin on UniProt.org
