Alexa Fluor® 647 anti-Nestin Antibody
- 产品名称：
- Alexa Fluor® 647 anti-Nestin Antibody
- 产品类别：
- 抗体
- 产品编号：
- 655107
- 产品应用：
- 655107
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Homogenized Sprague-Dawley rat spinal cord.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Tissue specificity of this Alexa Fluor? 647 anti-Nestin antibody was validated in embryonic day 15 mouse brain tissue.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The reactivity of Alexa Fluor? 647 anti-Nestin antibody was verified in FFPE embryonic mouse tissue.
Additional reported applications (for the relevant formats) include: immunofluorescence, immunohistochemistry, and immunoprecipitation1-4.
- Application References
(PubMed link indicates BioLegend citation) -
- Hockfield S, et al. 1985. J. Neurosci. 5:3310. (IP)
- Friedman B, et al. 1990. J. Comp. Neurol. 295:43. (IP)
- Brook GA, et al. 1994. Exp. Neurol. 126:31. (IP)
- Lendahl U, et al. 1990. Cell 60:585. (IP)
- Product Citations
-
- Feng H, et al. 2020. J Clin Invest. 130:6354. PubMed
- Ge H, et al. 2021. Front Mol Neurosci. 13:596039. PubMed
- RRID
- AB_2728489 (BioLegend Cat. No. 655107) AB_2728490 (BioLegend Cat. No. 655108)
- Structure
- Mouse nestin is 1864 amino acids with a predicted molecular weight of 207 kD. The observed molecular weight is larger than 250 kD.
- Distribution
-
Cytosol
- Function
- Nestin gene encodes a member of the intermediate filament, and it is required for survival and renewal of neural progenitor cells.
- Cell Type
- Neural Stem Cells
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Antigen References
-
1. Lendahl U, et al. 1990. Cell 60:585.
2. Gilyarov AV, et al. 2008. Neurosci. Behav. Physiol. 38:165.
3. Méndez-Ferrer S, et al. 2010. Nature 466:829.
4. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
- Gene ID
- 10763 View all products for this Gene ID
- UniProt
- View information about Nestin on UniProt.org
