Ultra-LEAF™ Purified anti-mouse/human CD45R/-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2702	1

Product Details

Verified Reactivity

Mouse, Human

Reported Reactivity

Cat

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Abelson murine leukemia virus-induced pre-B tumor cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
CyTOF? - Verified
IHC-F, IHC-P, IP, Activ - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro and in vivo modulation of B cell responses^2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^5,6, and spatial biology (IBEX)^14,15.

Application References (PubMed link indicates BioLegend citation)

Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
George A, et al. 1994. J. Immunol. 152:1014. (Activ)
Asensi V, et al. 1989. Immunology 68:204. (Activ)
Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
Chang C L-T, et al. 2007. J. Immunol. 178:6984.
Fazilleau N, et al. 2007. Nature Immunol. 8:753.
Lang GL, et al. 2008. Blood 111:2158. PubMed
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Formaglio P, et al. 2021. Immunity. 54:2724. PubMed

RRID

AB_2876406 (BioLegend Cat. No. 103269) AB_2832305 (BioLegend Cat. No. 103270)

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: B cells, T cell subset, NK cell subset
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: B cells, NK cells, T cells
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt: View information about CD45R on UniProt.org

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