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PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 Antib
产品名称：
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 Antib
产品类别：
抗体
产品编号：
103235
产品应用：
103235
[价格]
规格 价格 库存
25µg ￥ 1260 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  15. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Sasaki I, et al. 2012. Blood. 120:4733. PubMed
  2. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  3. Zenke S, et al. 2022. Nat Commun. 13:6459. PubMed
  4. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  5. Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed
  6. Kaku H, et al. 2014. J Immunol. 193:5904. PubMed
  7. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  8. Page N, et al. 2018. Immunity. 48:937. PubMed
  9. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  10. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  11. Kong S, et al. 2015. J Immunol. 195: 3685 - 3693. PubMed
  12. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  13. Feng J, et al. 2021. iScience. 24:103004. PubMed
  14. Pack AD, et al. 2021. Cell Reports. 36(8):109586. PubMed
  15. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  16. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  17. Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed
  18. Pan H, et al. 2020. Mol Psychiatry. . PubMed
  19. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  20. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  21. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  22. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  23. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  24. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  25. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  26. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  27. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  28. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  29. Rivadeneira DB, et al. 2020. Immunity. 51(3):548-560. PubMed
  30. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  31. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  32. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  33. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  34. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  35. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  36. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  37. Pereira J, et al. 2010. PLoS One. 5:e9277. PubMed
  38. Dong S, et al. 2021. Nature. 591:117. PubMed
  39. Wang JC, et al. 2022. Elife. 11:. PubMed
  40. Yang J, et al. 2020. Nature. 586:572. PubMed
  41. Leal AS, et al. 2019. Sci Rep. 5.286111111. PubMed
  42. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  43. Melissa M Remy et al. 2017. Cell host & microbe. 22(3):354-365 . PubMed
  44. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  45. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  46. Xing Y, et al. 2022. Cancer Lett. 529:100. PubMed
  47. Webb ER, et al. 2022. iScience. 25:104995. PubMed
  48. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  49. Hilpert C, et al. 2019. J Immunol. 203:3068. PubMed
  50. Beloor J et al. 2018. Cell host & microbe. 23(4):549-556 . PubMed
  51. Li Y, et al. 2021. Front Cell Dev Biol. 9:646077. PubMed
  52. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  53. Giampazolias E, et al. 2021. Cell. . PubMed
  54. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  55. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  56. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  57. Srinivasan A, et al. 2021. BMC Immunol. 22:46. PubMed
  58. Shibad V, et al. 2021. Front Immunol. 12:722451. PubMed
  59. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  60. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  61. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  62. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  63. He C, et al. 2022. Nat Commun. 13:5459. PubMed
  64. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  65. Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed
  66. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  67. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  68. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  69. Liu B, et al. 2013. Development. 140:780. PubMed
  70. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  71. Vijay R, et al. 2021. J Exp Med. 218:. PubMed
  72. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  73. Neckermann P, et al. 2021. Front Immunol. 12:761214. PubMed
  74. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  75. Zhang L, et al. 2020. Cell. 442:181. PubMed
  76. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  77. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  78. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
  79. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  80. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  81. Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
  82. Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
  83. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
  84. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  85. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  86. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  87. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
  88. Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
  89. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
RRID
AB_893356 (BioLegend Cat. No. 103235) AB_893354 (BioLegend Cat. No. 103236)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

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