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PE/Cyanine5 anti-mouse/human CD45R/B220 Antibody
产品名称：
PE/Cyanine5 anti-mouse/human CD45R/B220 Antibody
产品类别：
抗体
产品编号：
103209
产品应用：
103209
[价格]
规格 价格 库存
25µg ￥ 1078 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  15. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  3. Thelin MA, et al. 2017. Diabetes. 66:2220. PubMed
  4. Kinder JM, et al. 2020. Cell Reports. 31(12):107784.. PubMed
  5. Suzuki Y, et al. 2022. Mol Ther Nucleic Acids. 30:226. PubMed
  6. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  7. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  8. Uchida A, et al. 2021. J Inflamm Res. 14:3089. PubMed
  9. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  10. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  11. Nelson SA, et al. 2022. NPJ Vaccines. 7:124. PubMed
  12. Sodji QH, et al. 2022. Cancer Res Commun. 2:725. PubMed
  13. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  14. Dawar S, et al. 2016. Cell Death Dis. 7:e2509. PubMed
  15. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  16. Yankova E, et al. 2021. Nature. 593:597. PubMed
  17. Strid T, et al. 2009. Biochem Biophys Res Commun. 383:336. PubMed
  18. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  19. Jaako P, et al. 2011. Blood. 118:6087. PubMed
  20. Naik A, et al. 2015. J Immunol. 194:790. PubMed
  21. Finsterbusch M, et al. 2014. J Exp Med. 211:1307. PubMed
  22. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  23. Severance AL, et al. 2022. iScience. 25:104400. PubMed
  24. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  25. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  26. Dulken BW, et al. 2019. Nature. 571:205. PubMed
  27. Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed
  28. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  29. Dholakia J, et al. 2022. Gynecol Oncol. 164:170. PubMed
  30. Koide S, et al. 2022. iScience. 25:103603. PubMed
  31. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  32. S?wen P et al. 2018. eLife. 7 pii: e41258. PubMed
  33. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  34. Chavez JS, et al. 2022. Cells. 11:. PubMed
  35. Peltzer N, et al. 2018. Nature. 557:112. PubMed
  36. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  37. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  38. Trivedi S, et al. 2020. Elife. 9:00. PubMed
RRID
AB_312994 (BioLegend Cat. No. 103209) AB_312995 (BioLegend Cat. No. 103210)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

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