热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
Brilliant Violet 421™ anti-mouse/human CD45R
产品名称：
Brilliant Violet 421™ anti-mouse/human CD45R
产品类别：
抗体
产品编号：
103239
产品应用：
103239
[价格]
规格 价格 库存
125µL ￥ 2310 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  15. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  2. Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed
  3. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  4. Sano T, et al. 2021. Cell Reports. 36(8):109608. PubMed
  5. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  6. Chen J, et al. 2022. J Nanobiotechnology. 20:283. PubMed
  7. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  8. Powell WE, et al. 2018. Diabetologia. 61:1794. PubMed
  9. Utley A, et al. 2020. Cell Rep. 31:107815. PubMed
  10. Hiramoto T, et al. 2018. Mol Ther. 26:1255. PubMed
  11. Li J, et al. 2020. Elife. 9:00. PubMed
  12. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  13. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  14. Marchalot A, et al. 2020. Front Immunol. 11:780. PubMed
  15. Huang YL, et al. 2021. Commun Biol. 4:229. PubMed
  16. Liu X, et al. 2022. Nat Cancer. :. PubMed
  17. Lindner J, et al. 2014. J Immunol. 192:4483. PubMed
  18. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
  19. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  20. Siegemund S, et al. 2015. PLoS One. 10:124661. PubMed
  21. Argüello RJ, et al. 2018. J Cell Sci. 131:jcs214346. PubMed
  22. Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed
  23. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
  24. Bi E, et al. 2018. J Clin Invest. 128:4821. PubMed
  25. Zwick M, et al. 2019. Front Immunol. 10:222. PubMed
  26. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  27. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  28. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  29. Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
  30. Girón-Pérez DA, et al. 2020. J Cell Sci. 133:00:00. PubMed
  31. Koohy H, et al. 2018. Genome Biol. 0.879166667. PubMed
  32. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  33. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  34. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  35. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
  36. Lightman SM, et al. 2020. Cell Reports. 1.086805556. PubMed
  37. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  38. Yá?ez A et al. 2017. Immunity. 47(5):890-902 . PubMed
RRID
AB_10933424 (BioLegend Cat. No. 103239) AB_2562905 (BioLegend Cat. No. 103251) AB_11203896 (BioLegend Cat. No. 103240)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线