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Alexa Fluor® 700 anti-mouse/human CD45R/B220 A
产品名称：
Alexa Fluor® 700 anti-mouse/human CD45R/B220 A
产品类别：
抗体
产品编号：
103231
产品应用：
103231
[价格]
规格 价格 库存
25µg ￥ 1316 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45R antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  15. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed
  2. Yamin R, et al. 2021. Nature. 599:465. PubMed
  3. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  4. Sch?nberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  5. Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
  6. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  7. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  8. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  9. Shang Y, et al. 2016. Nat Immunol. 10.1038/ni.3486. PubMed
  10. Liu Y, et al. 2022. Nat Commun. 13:2665. PubMed
  11. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  12. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  13. Boyoglu-Barnum S, et al. 2014. J Virol. 88:10569. PubMed
  14. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  15. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  16. Ruf B, et al. 2021. Cancer Immunol Res. 9:1024. PubMed
  17. Agrawal M, et al. 2021. Cells. 10:. PubMed
  18. Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
  19. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  20. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  21. Peauroi EM, et al. 2022. iScience. 25:105510. PubMed
  22. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  23. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  24. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  25. Spix B, et al. 2022. Nat Commun. 13:318. PubMed
  26. Zhang Y, et al. 2022. Vaccines (Basel). 10:. PubMed
  27. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  28. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  29. Kumari P, et al. 2021. Cell Reports. 35(3):109012. PubMed
  30. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  31. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  32. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  33. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  34. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  35. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  36. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  37. Liu Q, et al. 2021. Adv Mater. 33:e2102852. PubMed
  38. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  39. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  40. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  41. Lee S, et al. 2021. Autophagy. Online ahead of print. PubMed
RRID
AB_493716 (BioLegend Cat. No. 103231) AB_493717 (BioLegend Cat. No. 103232)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

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