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Alexa Fluor® 647 anti-mouse/human CD45R/B220 A
产品名称：
Alexa Fluor® 647 anti-mouse/human CD45R/B220 A
产品类别：
抗体
产品编号：
103229
产品应用：
103229
[价格]
规格 价格 库存
25µg ￥ 1414 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified

SB -?Reported in the literature,?not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L?volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 ?g/mL?is suggested. For immunofluorescence microscopy, a concentration range of 1.25 - 10 ?g/mL?is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  15. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Caba?ero D, et al. 2020. Elife. 9:00. PubMed
  2. Wang Y, et al. 2021. Cancer Res. 81:174. PubMed
  3. Kim EH, et al. 2020. Elife. 9:00. PubMed
  4. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  5. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
  6. Kang S, et al. 2016. J Immunol. 196: 196 - 206. PubMed
  7. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  8. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  9. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  10. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  11. Brownlie R, et al. 2008. J Exp Med. 205:883. PubMed
  12. Bangs DJ, et al. 2022. Cell Rep. 38:110266. PubMed
  13. Neubert K, et al. 2014. J Immunol. 192:5830. PubMed
  14. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  15. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  16. Folgosa L, et al. 2013. J Immunol. 191:5951. PubMed
  17. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  18. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  19. Medgyesi D, et al. 2014. J Exp Med. 211:427. PubMed
  20. Emg?rd J, et al. 2018. Immunity. 143:419. PubMed
  21. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  22. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  23. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  24. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  25. Daniel CJ, et al. 2022. Mol Cancer Res. 20:1151. PubMed
  26. Yang J, et al. 2019. FASEB J. 33:12780. PubMed
  27. Lian J, et al. 2020. Cell Reports. 31(8):107679. PubMed
  28. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  29. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  30. Vogel AB, et al. 2021. Nature. 592:283. PubMed
  31. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  32. Tsai F, et al. 2017. J Exp Med. 214:3753. PubMed
  33. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  34. Hermetet F, et al. 2019. Nat Commun. 10:523. PubMed
  35. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  36. Ramakrishnan SK, et al. 2019. Nat Commun. 10:660. PubMed
  37. Chen C, et al. 2021. J Virol. 95:e0082921. PubMed
  38. Cheng HW, et al. 2022. Nat Commun. 13:2027. PubMed
  39. Gil-Cruz C, et al. 2012. Proc Natl Acad Sci U S A. 109:1233. PubMed
  40. Fleig S, et al. 2022. Nat Commun. 13:2022. PubMed
  41. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  42. Cao A, et al. 2018. Nat Commun. 9:3288. PubMed
  43. Prados A, Kollias G, Koliaraki V 2016. Sci Rep. 6: 33027. PubMed
  44. Smith LK, et al. 2021. Elife. 10:. PubMed
  45. Harumiya S, et al. 2013. Arch Biochem Biophys. 533:18. PubMed
  46. Dammeijer F, et al. 2020. Cancer Cell. 38(5):685-700.e8. PubMed
  47. Campisi L, et al. 2016. Nat Immunol. 10.1038/ni.3512. PubMed
  48. Yoshikawa S, et al. 2016. Sci Rep. 6:18738. PubMed
RRID
AB_492875 (BioLegend Cat. No. 103229) AB_389330 (BioLegend Cat. No. 103226)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

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