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Alexa Fluor® 594 anti-mouse/human CD45R/B220 A
产品名称：
Alexa Fluor® 594 anti-mouse/human CD45R/B220 A
产品类别：
抗体
产品编号：
103254
产品应用：
103254
[价格]
规格 价格 库存
100µg ￥ 3542 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
FC, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunohistochemistry, a concentration range of 2.5-5 μg/mL?is recommended. For immunofluorescence microscopy, a concentration range of 1.25 - 10 μg/mL?is recommended. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?L?volume.?For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  15. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  2. Li S, et al. 2020. Cell Death Dis. 0.9875. PubMed
  3. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  4. Counoupas C, et al. 2020. NPJ Vaccines. 0.28125. PubMed
  5. Matryba P, et al. 2020. J Immunol. 1395:204. PubMed
  6. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  7. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  8. Luo L, et al. 2021. J Neuroinflammation. 18:27. PubMed
  9. Shi H et al. 2018. Immunity. 49(5):899-914 . PubMed
  10. Goldberg MF et al. 2018. Immunity. 49(6):1090-1102 . PubMed
  11. Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed
  12. Bidet K, et al. 2019. NPJ Vaccines. 4:27. PubMed
  13. Song W, et al. 2022. Immunity. 55:290. PubMed
RRID
AB_2563229 (BioLegend Cat. No. 103254)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

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