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Alexa Fluor® 488 anti-mouse/human CD45R/B220 A
产品名称：
Alexa Fluor® 488 anti-mouse/human CD45R/B220 A
产品类别：
抗体
产品编号：
103228
产品应用：
103228
[价格]
规格 价格 库存
25µg ￥ 1414 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 μg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  15. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed
  2. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  3. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  4. Mostofa A, et al. 2021. JCI Insight. 6:. PubMed
  5. Ooi JD, et al. 2019. Nat Commun. 10:3392. PubMed
  6. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  7. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  8. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  9. Wang J, et al. 2018. Front Immunol. 9:371. PubMed
  10. Shimoda A, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.07.126. PubMed
  11. Schnoor M, et al. 2020. Bio Protoc. 10:e3842. PubMed
  12. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  13. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  14. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  15. Serger E, et al. 2022. Nature. 607:585. PubMed
  16. Lin J, Sorger M 2015. Sci Rep. 6: 8390. PubMed
  17. Luo Z, et al. 2013. J Control Release. 170:259. PubMed
  18. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  19. Ge S, et al. 2012. Nephrol Dial Transplant. 209:35:00. PubMed
  20. Wang C, et al. 2014. Vaccine. 32:5475. PubMed
  21. Frasch S, et al. 2016. J Immunol. 197: 1425 - 1434. PubMed
  22. Chen F et al. 2018. Cell reports. 25(10):2775-2783 . PubMed
  23. Si Y, et al. 2018. J Control Release. 282:120. PubMed
  24. Lian J, et al. 2020. Cell Reports. 31(8):107679. PubMed
  25. Uribesalgo I, et al. 2019. EMBO Mol Med. 11:e9266. PubMed
  26. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  27. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  28. Tsai F, et al. 2017. J Exp Med. 214:3753. PubMed
  29. Li N, et al. 2021. iScience. 24:103163. PubMed
  30. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  31. Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
  32. Hu M, et al. 2020. Cancer Immunol Res. 8:1150. PubMed
  33. INAGAWA H, et al. 2016. In Vivo. 30: 205 - 211. PubMed
  34. Tanaka Y, et al. 2017. J Immunol. 199:4016. PubMed
  35. Luo L, et al. 2021. J Neuroinflammation. 18:27. PubMed
  36. Girón-Pérez DA, et al. 2020. J Cell Sci. 133:00:00. PubMed
  37. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  38. Werner A, et al. 2021. iScience. 24:103076. PubMed
  39. Nunes NS, et al. 2019. EBioMedicine. 45:495. PubMed
  40. Chen B, et al. 2021. Cell. 184:6262. PubMed
  41. Ceglia V, et al. 2021. J Immunol. 207:2060. PubMed
  42. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  43. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
RRID
AB_492874 (BioLegend Cat. No. 103228) AB_389308 (BioLegend Cat. No. 103225)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

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