PE anti-rat TCR α/β Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1456	1

Product Details

Verified Reactivity

Rat

Reported Reactivity

Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Rat T cell blasts & erythrocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting³, immunohistochemical staining of acetone-fixed frozen sections¹, in vitro activation of α/β TCR⁺ T cells ^1,2 and in vivo depletion² of α/β TCR⁺ T cells . The LEAF? purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 201112).

Application References (PubMed link indicates BioLegend citation)

Hunig T, et al. 1989. J. Exp. Med. 169:73.
Mitnacht R, et al. 1995. Eur. J. Immunol. 25:328.
Brenan M, et al. 1997. Eur. J. Immunol. 27:3070.
Yoshino N, et al. 2000. Exp. Anim. 49:97.

RRID

AB_313916 (BioLegend Cat. No. 201107)

Antigen Details

Structure: Ig superfamily, CD3/TCR complex with CD3 and TCR-α subunit, 40 and 46 kD
Distribution: Most peripheral T cells, intestinal epithelial lymphocytes, thymocytes
Function: Antigen recognition, T cell activation/responses
Ligand/Receptor: Peptide bound-MHC class I and II
Cell Type: Lymphocytes, T cells, Thymocytes
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Hunig T, et al. 1989. J. Exp. Med. 169:73.
2. Fangmann J, et al. 1991. Eur. J. Immunol. 21:753.
3. Mitnacht R, et al. 1995. Eur. J. Immunol. 25:328.
4. Peiters RH, et al. 1995. Biochem. Biophys. Res. Commun. 214:552.
5. Brenan M, et al. 1997. Eur. J. Immunol. 27:3070.
Gene ID: 24820 View all products for this Gene ID 290071 View all products for this Gene ID
UniProt: View information about TCR alpha/beta on UniProt.org

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