首页 > 抗体蛋白 > 抗体
Purified anti-mouse IFN-γ Antibody
产品名称:
Purified anti-mouse IFN-γ Antibody
产品类别:
抗体
产品编号:
505701
产品应用:
505701
[价格]
规格 价格 库存
50µg ¥ 1162 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partially-purified, native mouse IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture1-4,6,10,11 or ELISPOT Capture5: The purified R4-6A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated XMG1.2 antibody (Cat. No. 505804) as the detecting antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture. For ELISPOT Capture, the suggested use of this antibody is 1.0 - 4.0 ?g/mL.
ELISA or ELISPOT Detection: The biotinylated R4-6A2 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified XMG1.2 antibody (Cat. Nos. 505802 & 505812) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard.
Neutralization1,2,9: The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. Nos. 505708 - 505713).
Additional reported applications (for the relevant formats) include: immunohistochemical staining3,6,8 of acetone-fixed frozen tissue sections.
Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend's ELISA Max? Sets (Cat. No. 430801 and 430804) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Stevenson M, et al. 1990. Infect. Immun. 58:3225.
  3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
  4. Yang X, et al. 1993. J. Immunoassay 14:129.
  5. Klinman D, et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.19.
  6. Sander B, et al. 1991. Immunol. Rev. 119:65.
  7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
  8. Khanna A, et al. 2000. J. Immunol. 164:1346.
  9. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349.
  10. Dzhagalov I, et al. 2007. J. Immunol. 178:2113.
  11. Xu G, et al. 2007. J. Immunol. 179:5358.
Product Citations
  1. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  2. Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed
  3. Singh R, et al. 2017. J Immunol. 10.4049/jimmunol.1602010. PubMed
  4. Dong S, et al. 2020. J Control Release. 328:653. PubMed
  5. Rauschenberger T, et al. 2019. Front Immunol. 10:1917. PubMed
  6. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  7. Lai L, et al. 2011. Cancer Res. 71:61. PubMed
  8. Godoy-Calderón MJ, et al. 2018. Oncotarget. 8:11370. PubMed
  9. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  10. Xu G, et al. 2007. J Immunol. 179:5358. PubMed
  11. Zhang Y, et al. 2022. Nat Commun. 13:4948. PubMed
  12. Chen YH, et al. 2022. Arthritis Res Ther. 24:27. PubMed
RRID
AB_315389 (BioLegend Cat. No. 505701) AB_315390 (BioLegend Cat. No. 505702)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
  2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
  3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
  4. Gray P, et al. 1987. Lymphokines 13:151.
Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

联系我们

TEL:021-34661275  点击拨打热线