上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 500µg | ￥ 3836 | 1 |
ELISA Detection, ELISPOT Detection
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein (Cat. No. 575309) are recommended at concentration up to 2000 pg/mL. The final standard range should be determined by the end user.
For use as an ELISPOT detection antibody, a concentration range of 1-4 ?g/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
ELISA Capture1-4,6,10,11 or ELISPOT Capture5: The purified R4-6A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated XMG1.2 antibody (Cat. No. 505804) as the detecting antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture. For ELISPOT Capture, the suggested use of this antibody is 1.0 - 4.0 ?g/mL.
ELISA or ELISPOT Detection: The biotinylated R4-6A2 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified XMG1.2 antibody (Cat. Nos. 505802 & 505812) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard.
Neutralization1,2,9: The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. Nos. 505708 - 505713).
Additional reported applications (for the relevant formats) include: immunohistochemical staining3,6,8 of acetone-fixed frozen tissue sections.
Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend's ELISA Max? Sets (Cat. No. 430801 and 430804) are specially developed and recommended.
