PE anti-mouse CD179a (VpreB) Antibody
- 产品名称：
- PE anti-mouse CD179a (VpreB) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 143603
- 产品应用：
- 143603
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Soluble mouse VpreB protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western Blotting1, immunoprecipitation1, immunofluorescence staining of 70Z/3 cells1, and ELISA1.
- Application References
(PubMed link indicates BioLegend citation) -
- Stephan R, et al. 2001. J. Immunol. 167:3734. (ELISA, IF, IP, WB)
- RRID
- AB_11147372 (BioLegend Cat. No. 143603) AB_11150774 (BioLegend Cat. No. 143604)
- Structure
- Ig superfamily, Ig V-like, 18 kD, associates with CD179b to form surrogate light chain
- Distribution
- Pro and pre-B cells
- Function
- Pre-BCR complex plays a critical role in B cell development
- Cell Type
- B cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stephan R, et al. 2001. J. Immunol. 167:3734.
2. Wang YH, et al. 2002. Blood 99:2459.
3. Martensson IL, et al. 2000. Immunology 101:435.
- Gene ID
- 22362 View all products for this Gene ID
- UniProt
- View information about CD179a on UniProt.org
