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Purified anti-GD3 (MEL-1) Antibody (Previously Cov
产品名称:
Purified anti-GD3 (MEL-1) Antibody (Previously Cov
产品类别:
抗体
产品编号:
917701
产品应用:
917701
[价格]
规格 价格 库存
1mL ¥ 5488 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against SK-MEL-28 melanoma cell line.
Formulation
Phosphate-buffered solution with BSA + 0.1% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
? IHC: ≥1:40 with for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: The preferred specimens for this antibody are frozen tissues and/or cytocentrifuge preparations fixed 10 minutes in 4°C acetone.
Pretreatment: Not required
Incubation: 20-60 minutes at room temperature

Application Notes

This antibody is effective in immunohistochemistry (IHC).

Positive tissue (human): Melanoma

MEL-1 (clone R24) recognizes disialoganglioside GD3, a prominent cell surface marker of human melanoma cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Meidenbauer N, et al. 2004. Cancer Res. 64: 6319.
  2. Kasahara K, et al. 2000. J. Biol. Chem. 275: 34701.
  3. Watarai S, et al. 1991. J. Biochem. 110: 889.
  4. Yuyama K, et al. 2007. J. Biol. Chem. 282: 26392.
Product Citations
  1. Buchholz DE, et al. 2020. Proc Natl Acad Sci U S A. 117:15085. PubMed
RRID
AB_2565200 (BioLegend Cat. No. 917701)

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