Purified anti-GD3 (MEL-1) Antibody (Previously Cov
- 产品名称:
- Purified anti-GD3 (MEL-1) Antibody (Previously Cov
- 产品类别:
- 抗体
- 产品编号:
- 917701
- 产品应用:
- 917701
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against SK-MEL-28 melanoma cell line.
- Formulation
- Phosphate-buffered solution with BSA + 0.1% NaN3.
- Preparation
- The antibody was purified by affinity chromatography.
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
IHC-F - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
? IHC: ≥1:40 with for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).
Tissue Sections: The preferred specimens for this antibody are frozen tissues and/or cytocentrifuge preparations fixed 10 minutes in 4°C acetone.
Pretreatment: Not required
Incubation: 20-60 minutes at room temperature
- Application Notes
This antibody is effective in immunohistochemistry (IHC).
Positive tissue (human): Melanoma
MEL-1 (clone R24) recognizes disialoganglioside GD3, a prominent cell surface marker of human melanoma cells.
- Application References
(PubMed link indicates BioLegend citation) -
- Meidenbauer N, et al. 2004. Cancer Res. 64: 6319.
- Kasahara K, et al. 2000. J. Biol. Chem. 275: 34701.
- Watarai S, et al. 1991. J. Biochem. 110: 889.
- Yuyama K, et al. 2007. J. Biol. Chem. 282: 26392.
- Product Citations
-
- Buchholz DE, et al. 2020. Proc Natl Acad Sci U S A. 117:15085. PubMed
- RRID
- AB_2565200 (BioLegend Cat. No. 917701)