Purified anti-human CD111 (Nectin-1) Antibody
- 产品名称:
- Purified anti-human CD111 (Nectin-1) Antibody
- 产品类别:
- 抗体
- 产品编号:
- 340402
- 产品应用:
- 340402
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Nectin-1 transfected NIH/3T3 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P - Verified
WB, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for relevant formats) include: immunoprecipitation, Western blot, immunohistochemical staining of frozen tissue sections, block HSV-1 and HSV-2 entry into cells.
- Application References
(PubMed link indicates BioLegend citation) -
- Cocchi F, et al. 1998. Proc. Natl. Acad. Sci. USA. 95:15700.
- Martinez WM and Spear PG. 2002. J. Virol. 76:7255.
- Reymond N,et al. 2004. J. Exp Med. 199:1331.
- RRID
- AB_2284683 (BioLegend Cat. No. 340402)
- Structure
- 75 kD type I transmembrane glycoprotein, Nectin family, Ig superfamily
- Distribution
-
Myelomonocytic cells, megakaryocytes, CD34-positive stem cells, endothelial cells, epithelial cells, and neuronal cells.
- Function
- Adhesion, receptor for Herpes simplex Virus 1 (HSV-1) and HSV-2
- Ligand/Receptor
- Nectin-1, -2, -3, and -4, CD155, HSV-1 and HSV-2
- Cell Type
- Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Megakaryocytes
- Biology Area
- Cell Biology, Immunology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Zola h, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication.
2. Simpson SA, et al. 2005. J. Neuroviral. 11:208.
3. Akhtar J, et al. 2008. Ophthalmol. Vis. Sci. 49:4026.
- Gene ID
- 5818 View all products for this Gene ID
- UniProt
- View information about CD111 on UniProt.org