Ultra-LEAF™ Purified anti-mouse CD49d Antibo-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2618	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

AKR/Cum mouse spontaneous T lymphoma line TK1

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, Block, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The R1-2 antibody has been shown to partially block CD49d mediated interactions and, in combination with the 9C10 (MFR4.B) antibody, completely blocks VCAM-1 binding to VLA-4. Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and T cell costimulation^1-4, immunoprecipitation¹, and immunohistochemistry of frozen sections⁵. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103629 and 103630).

Application References (PubMed link indicates BioLegend citation)

Holzmann B, et al. 1989. Cell 56:37. (IP, Block)
Ferguson TA, et al. 1993. J. Immunol. 150:1172. (Block)
Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
Kilshaw PJ, et al. 1991. Eur. J. Immunol. 21:2591. (Block)
Jaspers M, et al. 1995. Differentiation 59:79. (IHC)
Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)

RRID

AB_2832313 (BioLegend Cat. No. 103629) AB_2832314 (BioLegend Cat. No. 103630)

Antigen Details

Structure: Integrin family, associates with integrin β₁ or β₇, 150 kD
Distribution: T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells
Function: T cell costimulation, cell-cell and cell-matrix adhesion
Ligand/Receptor: VCAM-1, MAdCAM-1, fibronectin
Cell Type: B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994 J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.
Gene ID: 16401 View all products for this Gene ID
UniProt: View information about CD49d on UniProt.org

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