FITC anti-mouse CD49d Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1162	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

AKR/Cum mouse spontaneous T lymphoma line TK1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)

Application Notes

The R1-2 antibody has been shown to partially block CD49d mediated interactions and, in combination with the 9C10 (MFR4.B) antibody, completely blocks VCAM-1 binding to VLA-4. Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and T cell costimulation^1-4, immunoprecipitation¹, and immunohistochemistry of frozen sections⁵. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103629 and 103630).

Application References (PubMed link indicates BioLegend citation)

Holzmann B, et al. 1989. Cell 56:37. (IP, Block)
Ferguson TA, et al. 1993. J. Immunol. 150:1172. (Block)
Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
Kilshaw PJ, et al. 1991. Eur. J. Immunol. 21:2591. (Block)
Jaspers M, et al. 1995. Differentiation 59:79. (IHC)
Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)

Product Citations

D?britz J, et al. 2016. Sci Rep. 6:20584. PubMed
Cabrera-Mora M, et al. 2016. J Immunol. 197: 2748 - 2761. PubMed
Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed
Tomov V, et al. 2013. J Virol. 87:7015. PubMed
Santa S, et al. 2014. J Immunol. 193:4704. PubMed
Tejedor L, et al. 2015. Brain Behav Immun. . PubMed
Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
Buzzelli JN, et al. 2019. Am J Physiol Gastrointest Liver Physiol. 316:G251. PubMed
Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed

RRID

AB_313036 (BioLegend Cat. No. 103605) AB_313037 (BioLegend Cat. No. 103606)

Antigen Details

Structure: Integrin family, associates with integrin β₁ or β₇, 150 kD
Distribution: T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells
Function: T cell costimulation, cell-cell and cell-matrix adhesion
Ligand/Receptor: VCAM-1, MAdCAM-1, fibronectin
Cell Type: B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994 J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.
Gene ID: 16401 View all products for this Gene ID
UniProt: View information about CD49d on UniProt.org

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