| 规格 | 价格 | 库存 |
|---|---|---|
| 100µL | ¥ 3542 | 1 |
ICC - Quality tested
WB, IP - Verified
IEM - Reported in the literature, not verified in house
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration of 4.0 μg/ml (1:250 dilution) is recommended.?For Western blotting, the suggested use of this reagent is 1.0 - 2.0 ?g per ml (1:500-1:1000 dilution). For immunoprecipitation, the suggested use of this reagent is 1:50 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
This antibody is effective in immunoblotting, immunocytochemistry (ICC) and immunoprecipitation (IP).
*Predicted MW = 250 kD
This antibody recognizes NUP153 as well as two related nuclear pore complex proteins: NUP214 and p62. By immunofluorescence, QE5 labels the nuclear envelope of eukaryotic cells giving a punctate staining pattern.
?
Predicted MW is ~ 250 kD, observed MW is ~154 kD. Lower bands (~ 60 kD) maybe the tripartite structure of NUP153, which consists of a zinc finger domain flanked by large (60-70 kD) NH2- and COOH-terminal domains9.