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Purified anti-NUP153 Antibody (Previously Covance
产品名称:
Purified anti-NUP153 Antibody (Previously Covance
产品类别:
抗体
产品编号:
906201
产品应用:
906201
[价格]
规格 价格 库存
100µL ¥ 3542 1

产品详情

Product Details

Verified Reactivity
Eukaryote
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The QE5 monoclonal antibody was generated against rat liver nuclear envelope proteins.
Formulation
Phosphate-buffered solution + 0.03% thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

ICC - Quality tested
WB, IP - Verified
IEM - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration of 4.0 μg/ml (1:250 dilution) is recommended.?For Western blotting, the suggested use of this reagent is 1.0 - 2.0 ?g per ml (1:500-1:1000 dilution). For immunoprecipitation, the suggested use of this reagent is 1:50 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting, immunocytochemistry (ICC) and immunoprecipitation (IP).

*Predicted MW = 250 kD

This antibody recognizes NUP153 as well as two related nuclear pore complex proteins: NUP214 and p62. By immunofluorescence, QE5 labels the nuclear envelope of eukaryotic cells giving a punctate staining pattern.
?

Predicted MW is ~ 250 kD, observed MW is ~154 kD. Lower bands (~ 60 kD) maybe the tripartite structure of NUP153, which consists of a zinc finger domain flanked by large (60-70 kD) NH2- and COOH-terminal domains9.

Application References

(PubMed link indicates BioLegend citation)
  1. Pare GC, et al. 2005. Exp Cell Res. 303:388.
  2. N Pante, et al. 1994. J Cell Biol 126:603.
  3. Smythe C, et al. 2000. EMBO J. 19:3918. (WB)
  4. Goujon C, et al. 2008. J Virol. 82:12335. (IF) PubMed
  5. Holzenspies JJ, et al. 2009. BMC Dev Biol.9:8. (IF) PubMed
  6. Green JE, et al. 2014. Dev Biol. 392:419. (IF) PubMed
  7. Chen CY, et al. 2012. Cell. 149:565. (IF, WB)
  8. Iborra FJ, et al.2000. J Cell Sci. 113:291. (IEM)
  9. Bastos R, et al. 1997. J Cell Biol. 137:989. (IF, IP)
Product Citations
  1. H?lzenspies J, et al. 2009. BMC Dev Biol. 9:8. PubMed
  2. Goujon C, et al. 2008. J Virol. 82:12335-12345. PubMed
  3. Akam J 2014. Dev Biol. 392:419-430. PubMed
  4. Liu X et al. 2017. Cell. 170(5):1028-1043 . PubMed
  5. Shindo Y, et al. 2016. Nat Commun. 7:10485. PubMed
RRID
AB_2565060 (BioLegend Cat. No. 906201)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Nuclear Markers
Gene ID
9972 View all products for this Gene ID
UniProt
View information about NUP153 on UniProt.org

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