Alexa Fluor® 594 anti-NUP153 Antibody
- 产品名称：
- Alexa Fluor® 594 anti-NUP153 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 906204
- 产品应用：
- 906204
- Verified Reactivity
- Eukaryote
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- The QE5 monoclonal antibody was generated against rat liver nuclear envelope proteins.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
This antibody is effective in immunoblotting, immunofluorescence (IF) and immunoprecipitation (IP).
*Predicted MW = 250 kD
This antibody recognizes NUP153 as well as two related nuclear pore complex proteins: NUP214 and p62. By immunofluorescence, QE5 labels the nuclear envelope of eukaryotic cells giving a punctate staining pattern.
- Application References
(PubMed link indicates BioLegend citation) -
- Pare GC, et al. 2005. Exp Cell Res. 303:388.
- N Pante, et al. 1994. J Cell Biol. 126:603.
- Smythe C, et al. 2000. EMBO J. 19:3918. (WB)
- Goujon C, et al. 2008. J Virol. 82:12335. (IF) PubMed
- Holzenspies JJ, et al. 2009. BMC Dev Biol.9:8. (IF) PubMed
- Green JE, et al. 2014. Dev Biol. 392:419. (IF) PubMed
- Chen CY, et al. 2012. Cell. 149:565. (IF, WB)
- Iborra FJ, et al.2000. J Cell Sci. 113:291. (IEM)
- Bastos R, et al. 1997. J Cell Biol. 137:989. (IF, IP)
- RRID
- AB_2572062 (BioLegend Cat. No. 906204)
