Ultra-LEAF™ Purified anti-mouse IL-4 Recombi
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse IL-4 Recombi
- 产品类别：
- 抗体
- 产品编号：
- 942803
- 产品应用：
- 942803
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Recombinant mouse IL-4
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by the inhibition of proliferation induced by recombinant mouse IL-4 (Cat. No. 574306) at 7 ng/mL on mouse HT-2 cells. ND50 range: 0.1 - 0.5 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2910503 (BioLegend Cat. No. 942803) AB_2910503 (BioLegend Cat. No. 942804)
- Structure
- Heterodimer, interleukin
- Distribution
-
Th2 cells, NK T cells, mast cells, basophils, eosinophils, macrophages
- Function
- B-cell stimulatory cytokine, Th2 promoting, immunoglobulin isotype switching to IgG1 and IgE, upregulates MHC class II production, T-cell suppressor, regulator in humoral and adaptive immunity, decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL-12.
- Interaction
- T cells, B cells, mast cells, monocytes, macrophages, fibroblast, and endothelial cells
- Ligand/Receptor
- Type I receptor complex (IL-4Rα, γc) and type II receptor complex (IL-4Rα, IL-13Rα1)
- Cell Type
- Basophils, Embryonic Stem Cells, Eosinophils, Hematopoietic stem and progenitors, Macrophages, Mast cells, NKT cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Chen. L, et al. 2004. J. Immunol. 172:2059.
- Maroof A, et al. 2006. Immunology 117:271.
- Sharma P, et al. 2008. Immunity 29:551.
- Heller NM, et al. 2008. Sci. Signal 1:ra17.
- LaPorte SL, et al. 2008. Cell 132:259.
- Perrigoue JG, et al. 2009. Nat. Immunol. 10:697.
- Schneider E, et al. 2010. Eur. Cytokine Netw. 21:142.
- Leavy O. 2011. Nat. Rev. Immunol. doi:10.1038/nri2908.
- Gene ID
- 16189 View all products for this Gene ID
- UniProt
- View information about IL-4 on UniProt.org
