PE anti-mouse CD45 Recombinant Antibody
- 产品名称：
- PE anti-mouse CD45 Recombinant Antibody
- 产品类别：
- 抗体
- 产品编号：
- 157603
- 产品应用：
- 157603
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The QA17A26 antibody can block 30-F11 clone? and vice versa. The clone QA17A26 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.
- Application References
(PubMed link indicates BioLegend citation) -
- Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
- Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
- Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
- Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
- Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
- Cornet A, et al. 2001. Proc. Natl. Acad. Sci. USA 98:13306. (IHC)
- Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
- Liu F, et al. 2012. Blood. 119:3295. PubMed
- Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
- RRID
- AB_2876536 (BioLegend Cat. No. 157603) AB_2876536 (BioLegend Cat. No. 157604)
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
Expressed on almost all hematopoietic cells except for mature erythrocytes.
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
- Cell Type
- B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay AN,?et al.?1997. The Leukocyte Antigen FactsBook Academic Press.
- Trowbridge IS and Thomas ML. 1994. Annu. Rev. Immunol. 12:85-116.
- Kishihara K,?et al.?1993.?Cell?74:143.
- Pulido R,?et al.?1988.?J. Immunol.?140:3851.
?
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
