Alexa Fluor® 700 anti-mouse CD45 Recombinant A
- 产品名称：
- Alexa Fluor® 700 anti-mouse CD45 Recombinant A
- 产品类别：
- 抗体
- 产品编号：
- 157615
- 产品应用：
- 157615
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The QA17A26 antibody can block 30-F11 clone? and vice versa. The clone QA17A26 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.
- Application References
(PubMed link indicates BioLegend citation) -
- Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
- Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
- Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
- Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
- Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
- Cornet A, et al. 2001. Proc. Natl. Acad. Sci. USA 98:13306. (IHC)
- Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
- Liu F, et al. 2012. Blood. 119:3295. PubMed
- Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
- RRID
- AB_2890719 (BioLegend Cat. No. 157615) AB_2890719 (BioLegend Cat. No. 157616)
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
Expressed on almost all hematopoietic cells except for mature erythrocytes.
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
- Cell Type
- B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay AN,?et al.?1997. The Leukocyte Antigen FactsBook Academic Press.
- Trowbridge IS and Thomas ML. 1994. Annu. Rev. Immunol. 12:85-116.
- Kishihara K,?et al.?1993.?Cell?74:143.
- Pulido R,?et al.?1988.?J. Immunol.?140:3851.
?
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
