TotalSeq™-D0168 anti-human CD57 Recombinant -上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 5166	1

Product Details

Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Membrane extract of human lymphoblastoid cell line HSB-2.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation: The antibody was purified by chromatography and conjugated with TotalSeq?-D oligomer under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: PG - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by?immunofluorescent staining with flow cytometric analysis?and the oligomer sequence is confirmed by sequencing. TotalSeq?-D antibodies are compatible with?Mission Bio’s Tapestri Single-Cell Sequencing Platform?for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension.?For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume.?Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.
Additional Product Notes: TotalSeq?-D reagents are designed to profile protein expression at single cell level. The?Mission Bio Tapestri platform?and sequencer (e.g. Illumina analyzers) are required. Please contact?technical support?for more information, or visit?biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our?Application Technical Notes.
RRID: AB_2894666 (BioLegend Cat. No. 393333)

Antigen Details

Structure

Oligosaccharide antigenic determinant present on a variety of proteins, lipds, and chrondroitin sulfate proteoglycans, 100-115 kD. The antigen is convserved across species.

Distribution

NK cells, subset of CD8+ T cells, neural cells, striated muscle

Ligand/Receptor

Binds to L-selectin and P-selectin in a calcium-dependent manner, also binds to second globular domain of E8 laminin fragment.

Cell Type

NK cells, T cells

Biology Area

Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology

Molecular Family

CD Molecules

Antigen References

Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711-714.
Palmer BE, et al. 2005. J. Immunol. 175:8415.
Schachner M, et al. 1995. Trends Neurosci. 18:183.
Wood KL, et al. 2005. Clin. Immunol. 117:294.

Gene ID