首页 > 抗体蛋白 > 抗体
Purified anti-Phosphotyrosine Antibody
产品名称:
Purified anti-Phosphotyrosine Antibody
产品类别:
抗体
产品编号:
309301
产品应用:
309301
[价格]
规格 价格 库存
25µg ¥ 756 1

产品详情

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated phosphotyrosine
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored at -20°C.
Application

WB - Quality tested
ICC - Verified
ICFC, IP- Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Suggested working dilution(s): Use 0.125 - 1.0 ?g per ml antibody dilution buffer per mini-gel. Do not use dilution or blocking buffers containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody. For immunocytochemistry, a concentration range of 0.2 - 0.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
  2. Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
  3. Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (ICC)
  4. Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
  5. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
  6. Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed
Product Citations
  1. Prochazka R, et al. 2012. Reproduction. 144:535. PubMed
  2. Céspedes P, et al. 2014. Proc Natl Acad Sci U S A. 111:3214. PubMed
  3. Zentilin L, et al. 2009. FASEB J. 24:1467. PubMed
RRID
AB_314763 (BioLegend Cat. No. 309301) AB_314764 (BioLegend Cat. No. 309302)

Antigen Details

Distribution

Phospho-Specific

Function
Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Gene ID
5800 View all products for this Gene ID
UniProt
View information about Phosphotyrosine on UniProt.org

联系我们

TEL:021-34661275  点击拨打热线