Biotin anti-Phosphotyrosine Antibody
- 产品名称:
- Biotin anti-Phosphotyrosine Antibody
- 产品类别:
- 抗体
- 产品编号:
- 309304
- 产品应用:
- 309304
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated phosphotyrosine
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.?Suggested working dilution(s): Use 5 ?g/5ml antibody dilution buffer per mini-gel. Do not use dilution or blocking buffers containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunocytochemistry3.
- Application References
(PubMed link indicates BioLegend citation) -
- Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
- Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
- Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (IF)
- Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
- Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
- Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed
- Product Citations
-
- Mukherjee K, et al. 2020. Front Immunol. 11:332. PubMed
- RRID
- AB_314766 (BioLegend Cat. No. 309304)