Alexa Fluor® 647 anti-Phosphotyrosine Antibody-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3710	1

Product Details

Verified Reactivity

Human, Mouse, Rat, All Species

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

KLH-conjugated phosphotyrosine

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 ?g per 10⁶ cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.

NOTE: Do not use dilution buffer containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, Western blotting^1,2, immunocytochemistry³.

Application References (PubMed link indicates BioLegend citation)

Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (IF)
Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed

Product Citations

Ma F, et al. 2012. J Biol Chem. 287:38073. PubMed
Martinez-Martin N, et al. 2016. Nat Commun. 7:11473. PubMed
Ramello M, et al. 2014. Cell Death Dis. 6:1507. PubMed

RRID

AB_528951 (BioLegend Cat. No. 309308)

Antigen Details

Distribution: Phospho-Specific
Function: Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Gene ID: 5800 View all products for this Gene ID
UniProt: View information about Phosphotyrosine on UniProt.org

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