Alexa Fluor® 488 anti-Phosphotyrosine Antibody
- 产品名称：
- Alexa Fluor® 488 anti-Phosphotyrosine Antibody
- 产品类别：
- 抗体
- 产品编号：
- 309306
- 产品应用：
- 309306
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated phosphotyrosine
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunocytochemistry3.
- Application References
(PubMed link indicates BioLegend citation) -
- Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
- Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
- Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (IF)
- Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
- Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
- Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed
- Product Citations
-
- Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
- Cassioli C, et al. 2021. Front Cell Dev Biol. 9:673446. PubMed
- RRID
- AB_528949 (BioLegend Cat. No. 309306)
