Purified anti-Presenilin 2 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1568	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This monoclonal antibody was raised using a GST-fusion containing the complete N-terminal fragment of human Presenilin 2 (residues 1-87).

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

WB -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1?- 10??g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Cell type: SH-SY5Y neuroblastoma cells. Presenilin 2 is proteolytically cleaved to generate two polypeptides. The full-length PS2 protein (~55 kD)?is cleaved to a ~ 35 kD N-terminal fragment and ~20 kD C-terminal fragment. The predominant species observed in cultured mammalian cells and in the brain is the processed fragment of ~35 kD. This antibody recognizes the full length and the ~35 kD cleavage fragment.

Primary antibody incubation period for WB: overnight at 4°C .

RRID

AB_2650835 (BioLegend Cat. No. 814203) AB_2650836 (BioLegend Cat. No. 814204)

Antigen Details

Structure: Presenilin 2 is a 448 amino acid protein with a molecular mass of ~ 55 kD.
Distribution: Tissue distribution: Presenilin 2 is ubiquitously expressed in humans. In the central nervous system (CNS), it is expressed by almost all cell types including astrocytes, neurons, microglia, unmyelinating and myelinating oligodendrocytes and progenitor cells.

Cellular distribution: Cytoskeleton, endoplasmic reticulum, nucleus and plasma membrane.
Function: Presenilin 2 helps process proteins that transmit chemical signals from the cell membrane into the nucleus. Once in the nucleus, these signals turn on (activate) genes that are important for cell growth and maturation. PS2 is best known for its role in processing amyloid precursor protein, which is found in the brain and other tissues. It has been suggested that presenilin 2 works together with other enzymes to cut amyloid precursor protein into smaller segments (peptides). One of these peptides is called soluble amyloid precursor protein (sAPP), and another is called amyloid beta peptide. sAPP has growth-promoting properties and may play a role in the formation of neurons in the brain both before and after birth.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Secretases
Antigen References: 1.?Brunello L,?et al.?2009.?J. Cell Mol. Med.?13:3358.?(WB)?PubMed
2.?Hutton M, Hardy J. 1997.?Hum. Mol. Genet.?6:1639.
Gene ID: 5664 View all products for this Gene ID
UniProt: View information about Presenilin 2 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们