Purified anti-Keratin 14 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 4900	1

Product Details

Verified Reactivity

Human, Mouse, Rat, Canine, Primate

Antibody Type

Polyclonal

Host Species

Chicken

Immunogen

This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 14 protein.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

IHC-P -?Quality tested
ICC, WB -?Verified
SB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemisty, a concentration of 1.0 μg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/mL is recommended.?For western blotting, the suggested usage is 1.0 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: spatial biology (IBEX)^1,2.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References (PubMed link indicates BioLegend citation)

Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed
Ding X, et al. 2016. Nat Commun. 7:13226. PubMed
Wong W, et al. 2020. PLoS One. 15:e0241617. PubMed
O'Rourke BP, et al. 2019. Adv Wound Care (New Rochelle). 8:91. PubMed
Redondo JA, et al. 2021. Biology (Basel). 10: . PubMed
Tripurani SK, et al. 2018. Mol Biol Cell. 29:2784. PubMed
Lepletier A et al. 2019. Cell Rep. 27(13):3887-3901 . PubMed
Yang K, et al. 2022. J Clin Invest. 132:. PubMed
Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
Meyer Zu Reckendorf S, et al. 2022. J Neurosci. 42:2474. PubMed
Stewart TA, et al. 2020. J Cell Sci. . PubMed
Frech S, et al. 2021. J Invest Dermatol. . PubMed
Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
Hu M, et al. 2019. Nat Commun. 10:3031. PubMed
Liu F, et al. 2021. Cell Reports. 35(10):109225. PubMed
Zhao W, et al. 2022. Bioeng Transl Med. 7:e10303. PubMed
Stewart TA, et al. 2020. J Cell Sci. 134:jcs248849. PubMed

RRID

AB_2616962 (BioLegend Cat. No. 906004)

Antigen Details

Structure

Keratin 14 is a 52 kD protein.

Distribution

Cytoplasm, extracellular, and nucleus.

Function

Nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles, enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

Biology Area

Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers

Molecular Family

Intermediate Filaments

Antigen References