Product Details

Product Information

Intended Use:

In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The polyclonal antibody Poly9059 is used for the in vitro examination of paraffin-embedded human skin tissue sections?using immunohistochemistry (IHC) methods for the qualitative identification of Keratin 5. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Reactivity

Human

Immunogen

This polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 5 protein.

Formulation

Phosphate-buffered solution + 0.1% sodium azide.

Preparation

The antibody was purified by IgY affinity chromatography.

Concentration

1.0 mg/mL

Storage & Handling

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining of formalin-fixed paraffin-embedded sections of human skin.

The optimal working dilution should be determined for each specific assay condition.
? IHC: 1:200 -1:1,000 with a biotin based detection system such as biotinylated anti-chicken immunoglobulin and USA Ultra Streptavidin Detection (Cat. No. 929501).

Positive Control tissue: Human skin

Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Sodium Citrate H.I.E.R Retrieval solution (Cat. No. 928502).
Incubation: 60 minutes at room temperature

Application References

(PubMed link indicates BioLegend citation)

1. Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
2. Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.

RRID

AB_2565054 (BioLegend Cat. No. 905901)

Disclaimer

WARNINGS AND PRECAUTIONS

1. Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
2. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
3. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
4. Do not use this reagent beyond the expiration date stated on the label.
5. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.