Purified anti-DYKDDDDK Epitope Tag Antibody (Previ
- 产品名称:
- Purified anti-DYKDDDDK Epitope Tag Antibody (Previ
- 产品类别:
- 抗体
- 产品编号:
- 902401
- 产品应用:
- 902401
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Polyclonal antibody DYKDDDDK was raised against the sequence CDYKDDDDK.
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB - Quality tested
ICC - Verified
Purification - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g/mL.?For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody is effective in Western Blotting (WB) and Immunocytochemistry (ICC) of tagged proteins.
This antibody recognizes the FLAG? epitope tag. The DYKDDDDK antibody does not require Ca++ for binding. It reacts with both N-terminal and C-terminal fusion proteins.
This product may contain other non-IgG subtypes
- Product Citations
-
- Rogers EJ, et al. 2019. Front Mol Neurosci. 12:43. PubMed
- Li Y, et al. 2008. J Biol Chem. 283:22443-22456. PubMed
- RRID
- AB_2565020 (BioLegend Cat. No. 902401)