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Purified anti-HA.11 Epitope Tag Antibody (Previous
产品名称：
Purified anti-HA.11 Epitope Tag Antibody (Previous
产品类别：
抗体
产品编号：
902303
产品应用：
902303
[价格]
规格 价格 库存
25µL ￥ 1246 1

产品详情

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Polyclonal antibody HA.11 was raised against the twelve amino acid sequence CYPYDVPDYASL.
Formulation
Phosphate-buffered solution + 0.03% thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IP
ChIP, ICC, Purification - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
? WB: 1:1,000*
? IP: 1:150

Application Notes

This antibody is effective in immunoblotting (WB) and immunoprecipitation (IP) of tagged proteins.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This antibody recognizes the same hemagglutinin flu epitope YPYDVPDYA as the monoclonal antibody, HA.11 clone 16B12. This polyclonal antibody facilitates epitope tag experiments in murine systems and is useful in experiments involving two proteins labeled with two different tags.

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Barrott JJ, et al. 2011. PNAS. 108: 12752. (WB, IP)
  2. Pecot MY, Malhotra V. 2004. Cell. 116:99.
  3. Delmas C, et al. 2003. J Biol Chem. 278:12443. (IF)
  4. Caplan S, et al. 2001. J Cell Biol. 154:109. (IF, WB)
  5. Yeo C, Whitman M. 2001. Mol Cell 7:949.
  6. Sander TL, et al. 2000. J Biol Chem.:12857. (WB)
  7. Bosshart H, et al. 1994. J Cell Biol. 126:1157. (IF, IP)
  8. Hewawasam G, et al. 2010. Mol Cell. 40:444. (ChIP, WB) PubMed
  9. Hurov JB, et al. 2004. Curr Biol. 14:736. (IF)
  10. Shieh AW, et al. 2013. PLoS One. 8:75337. (WB, IF) PubMed
  11. Hackenbeck T, et al. 2009. Cell Cycle. 8:1386. (ICC)
  12. Satheshkumar PS, et al. 2013. J Virol. 87:10710.
  13. Luo R, et al. 2009. Cell Signal. 21:1169. (WB) PubMed
  14. Berg TJ, et al. 2010. J Biol Chem. 285:35255. (WB) PubMed
Product Citations
  1. Chalker A 2013. PLoS One. 8:e75337. PubMed
  2. Rinschen M, et al. 2016. Hum Mol Genet. 25: 1328-1344. PubMed
  3. Luo R, et al. 2009. Cell Signal. 21:1169-1179. PubMed
  4. Berg T, et al. 2010. J Biol Chem. 285:35255-32566. PubMed
  5. Pal D, et al. 2022. Commun Biol. 5:646. PubMed
  6. Morales L, et al. 2022. Front Neural Circuits. 16:831074. PubMed
  7. Ho TYH, et al. 2021. Nat Commun. 12:2200. PubMed
  8. Tsao N, et al. 2016. Biochem J. 473: 1237 - 1245. PubMed
  9. Wang Q, et al. 2019. Biomolecules. 9:4. PubMed
  10. Liu X, et al. 2021. Nat Commun. 12:4427. PubMed
  11. Martin CK, et al. 2022. Viruses. 14:. PubMed
  12. Nissim S, et al. 2019. Nat Genet. 51:1308. PubMed
  13. Dafinger C, et al. 2018. Exp Mol Med. 50:75. PubMed
  14. Szilágyi SS, et al. 2022. BMC Biol. 20:50. PubMed
  15. Judith D, et al. 2019. J Cell Biol. 218:1634. PubMed
  16. Bartram M, et al. 2016. Hum Mol Genet. 25: 1152 - 1164. PubMed
  17. Hewawasam G, et al. 2010. Mol Cell. 40:444-454. PubMed
  18. Choi Y, et al. 2019. Nat Microbiol. 4:429. PubMed
  19. Choi YJ, et al. 2019. Nat Immunol. 20:276. PubMed
  20. Coulombe P et al. 2019. Cell reports. 27(6):1769-1780 . PubMed
  21. Robles‐Valero J et al. 2017. Cancer cell. 32(5):608-623 . PubMed
  22. Patchett S, et al. 2021. Cell Rep. 36:109754. PubMed
RRID
AB_2734670 (BioLegend Cat. No. 902303) AB_2565018 (BioLegend Cat. No. 902301) AB_2565019 (BioLegend Cat. No. 902302)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

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