Anti-HA Tag Antibody (Previously Covance catalog#
- 产品名称：
- Anti-HA Tag Antibody (Previously Covance catalog#
- 产品类别：
- 抗体
- 产品编号：
- 923501
- 产品应用：
- 923501
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Polyclonal antibody HA.11 was raised against the twelve amino acid sequence CYPYDVPDYASL.
- Preparation
- Serum
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
- Application
-
WB, IP
- Recommended Usage
The optimal working dilution should be determined for each specific assay condition.
? WB: 1:1,000*
? IP: 1:150
- Application Notes
This antibody is effective in immunoblotting (WB) and immunoprecipitation (IP) of tagged proteins.
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
This antibody recognizes the same hemagglutinin flu epitope YPYDVPDYA as the monoclonal antibody, HA.11 clone 16B12. This polyclonal antibody facilitates epitope tag experiments in murine systems and is useful in experiments involving two proteins labeled with two different tags.
This product may contain other non-IgG subtypes.
- Application References
(PubMed link indicates BioLegend citation) -
- Barrott JJ, et al. 2011. PNAS. 108: 12752. (WB, IP)
- Pecot MY, Malhotra V. 2004. Cell. 116:99.
- Delmas C, et al. 2003. J Biol Chem. 278:12443. (IF)
- Caplan S, et al. 2001. J Cell Biol. 154:109. (IF, WB)
- Yeo C, Whitman M. 2001. Mol Cell. 7:949.
- Sander TL, et al. 2000. J Biol Chem. 275:12857. (WB)
- Bosshart H, et al. 1994. J Cell Biol. 126:1157. (IF, IP)
- Product Citations
-
- Khan I, et al. 2021. Methods Mol Biol. 2262:281. PubMed
- Zhu Z, et al. 2016. J Virol. 90(24):11106-11121. PubMed
- Tazat K, et al. 2021. Mol Biol Cell. 32:605. PubMed
- Hao L, et al. 2021. Cell Prolif. :e12994. PubMed
- Zeng J, et al. 2020. Cell Stem Cell. 27(4):618-632.e9. PubMed
- Villeneuve J, et al. 2017. Mol Biol Cell. 28(1):141-151. PubMed
- Ting Y, et al. 2017. J Biol Chem. 292(2):585-596. PubMed
- Khan I, et al. 2022. Cell Rep. 38:110322. PubMed
- Trautz B, et al. 2016. J Virol. 90(23):10915-10927. PubMed
- Li J, et al. 2021. Med (N Y). 0.152083333. PubMed
- Wang S, et al. 2022. Methods Mol Biol. 2473:181. PubMed
- RRID
- AB_2565437 (BioLegend Cat. No. 923501) AB_2565438 (BioLegend Cat. No. 923502)
