Purified anti-β-actin Antibody
- 产品名称：
- Purified anti-β-actin Antibody
- 产品类别：
- 抗体
- 产品编号：
- 622101
- 产品应用：
- 622101
[价格]
| 规格 |
价格 |
库存 |
| 50µl(5Westernblots) |
￥ 1652 |
1 |
- Verified Reactivity
- Mouse, Rat, Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Peptide-KLH, NH2 terminus
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Upon receipt, store frozen at -20°C.
- Application
-
WB - Quality tested
ICC, IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 ?L?per 5 mL?antibody dilution buffer for each mini-gel. For immunocytochemistry: Use a dilution range of 1:100~1:400. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC?using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This product may contain other non-IgG subtypes.
- Application References
(PubMed link indicates BioLegend citation) -
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Joyce CW. 2006. J. Biol. Chem. 281:33053.
- Yanagiya T,et al.2007.Obesity.15:572.PubMed
- KishidaT,et al.2007.J. Immunol. 179:8554.PubMed
- Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144. PubMed
- Toltlt LJ,et al. 2008.J. Immunol. 181:2165. PubMed
- Sawada. T,et al. 2008. J. Biol. Chem. 283:26820. PubMed
- Ikeda D, et al. 2008. Endocrinology. 149:6037. PubMed
- Rahman MK, et al. 2010. J. Immunol. 184:7247. PubMed
- Shikama Y, et al. 2011. Innate Immun. 17:3. PubMed
- Zhang Y, et al. 2012. Am J Physiol Renal Physiol. 302:70. PubMed
- Piao X, et al. 2012. Sci Signal. 5:93. PubMed
- Ng CS, et al. 2013. J. Virol. 87:9511. PubMed
- Harvey BS, et al. 2014. Cytokine. 65:236. PubMed
- Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed
- Hua X, et al. 2015. PloS One. 10:128039. PubMed
- Product Citations
-
- Checa M, et al. 2016. PLoS One. 11: 0150383. PubMed
- Okimoto T, et al. 2020. Cancer Sci. 111:1910. PubMed
- Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed
- Al–Naggar IM, et al. 2019. J Gerontol A Biol Sci Med Sci. 74:325. PubMed
- Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
- Mete B, et al. 2022. Life Sci Alliance. 5:. PubMed
- Agabiti S, Li J, Wiemer A 2017. Cell Death Dis. 10.1038/cddis.2017.101. PubMed
- Wang D, et al. 2018. Immunity. 48:659. PubMed
- Piao X, et al. 2012. Sci Signal. 5:93. PubMed
- Wakatsuki S, et al. 2021. J Cell Biol. 220:. PubMed
- Harvey B, et al. 2014. Cytokine. 65:236. PubMed
- Wakatsuki S, et al. 2015. J Cell Biol. 211: 881 - 896. PubMed
- Kartika ID, et al. 2021. Cancer Med. 10:8988. PubMed
- Pfeil EM, et al. 2020. Molecular Cell. 80(6):940-954.e6. PubMed
- Hua X, et al. 2015. PLoS One. 10:128039. PubMed
- Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
- Takeda K, et al. 2017. Nat Commun. 8:14607. PubMed
- Che L, et al. 2019. Exp Ther Med. 18:1745. PubMed
- Ouimet M, et al. 2008. Arterioscler Thromb Vasc Biol. 28:1144. PubMed
- Rahman M, et al. 2010. J Immunol. 184:7247. PubMed
- Taha A, et al. 2020. J Cell Physiol. 7094:235. PubMed
- Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
- Toltl L, et al. 2008. J Immunol. 181:2165. PubMed
- Tao HC, et al. 2020. Front Immunol. 11:148. PubMed
- Man F, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L803. PubMed
- Berdasco C, et al. 2020. Front Cell Infect Microbiol. 0.681944444. PubMed
- Feddersen CR, et al. 2019. Cancer Res. 79:5074. PubMed
- Joyce C, et al. 2006. J Biol Chem. 281:33053. PubMed
- Zhu S et al. 2017. Cancer cell. 32(3):310-323 . PubMed
- Naneix F et al. 2017. eNeuro. 4(3) pii: ENEURO. PubMed
- Yanagiya T, et al. 2007. Obesity . 1.022222222. PubMed
- Kishida T, et al. 2007. J Immunol. 179:8554. PubMed
- Zheng X, et al. 2018. Int J Mol Med. 128:1300. PubMed
- Zhang Y, et al. 2012. Am J Physiol Renal Physiol. 302:F70. PubMed
- Terrazas M 2014. Int J Parasit. 44 613 . PubMed
- Ikeda D, et al. 2008. Endocrinology. 149:6037. PubMed
- Caglar Beker M, et al. 2020. Turk J Med Sci. Online ahead of print. PubMed
- Ng C, et al. 2013. J Virol. 87:9511. PubMed
- Fliegauf M, et al. 2021. Front Immunol. 12:621503. PubMed
- Grundmann M, et al. 2018. Nat Commun. 9:341. PubMed
- Sawada T, et al. 2008. J Biol Chem. 283:26820. PubMed
- Pototskiy E, et al. 2021. Sci Rep. 11:17262. PubMed
- Tsurusaki S, et al. 2019. Cell Death Dis. 10:449. PubMed
- Panwar P, et al. 2018. Br J Pharmacol. 175:902. PubMed
- Merten N et al. 2018. Cell chemical biology. 25(6):775-786 . PubMed
- Kumarasinghe N, et al. 2019. BMC Res Notes. 12:33. PubMed
- Inao T, et al. 2019. Cancer Sci. 110:2690. PubMed
- Shikama Y, et al. 2011. Innate Immunity. 17:03. PubMed
- Ergünay T, et al. 2022. Life Sci Alliance. 5:. PubMed
- RRID
- AB_315945 (BioLegend Cat. No. 622101) AB_315946 (BioLegend Cat. No. 622102)
- Structure
- Member of the actin family; 42 KD
- Distribution
-
Ubiquitously expressed in the cytoplasm of all eukaryotic cells
- Function
- Actins are highly conserved proteins that are involved in cell motility
- Interaction
- Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) that forms a two-stranded helix. Each actin can bind to four others.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microfilaments
- Antigen References
-
1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.
- Regulation
- Three actin isoforms exist in vertebrates; alpha, found in muscle tissues; beta and gamma, found as cytoskeletal components and mediators of internal cell motility. Drugs such as cytochalasin disrupt the actin network.
- Gene ID
- 11461 View all products for this Gene ID
- UniProt
- View information about beta-actin on UniProt.org
