Purified anti-human LIF Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 4900	1

Product Details

Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Human LIF, amino acids (Ser23-Phe202) (Accession# NP_002300.1), was expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. A concentration of 0.5 ?g/ml of the capture antibody was utilized to generate the example standard curve for human LIF.?For western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Capture: To measure human LIF, this antibody can be used as a capture antibody in a sandwich ELISA format and paired with the biotinylated clone M017C3 antibody as the detection antibody. Recombinant Human LIF (ELISA Std.) can be used as the protein standard.

Note: For testing human LIF in serum, plasma, or supernatant, BioLegend’s LEGEND MAX? Human LIF ELISA Kit is specially developed and recommended.
RRID: AB_2562322 (BioLegend Cat. No. 523301)

Structure: Four-helix bundle fold.
Distribution: Expressed in the trophectoderm of the developing embryo and activated CD4⁺ T cells.
Function: LIF is a pleiotropic cytokine involved in cellular development, inflammation, immunology, and neoplasia.
Interaction: Embryonic stem cells.
Ligand/Receptor: LIF Rα, gp130
Cell Type: T cells
Biology Area: Cell Biology, Immunology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Metcalf D. 1991. Int. J. Cell Cloning 2:95-108.
2. Zhang JG, et al. 1998. J. Biol. Chem. 17:10798.
3. Kamohara H, et al. 2007. Int. J. Oncol. 4:977.
4. Marshall MK, et al. 1994. Endocrinology 135:141.
5. Vogiagis D, Salamonsen LA. 1999. J. Endocrinol. 160:181.
6. Zouein FA, et al. 2013. Eur. Cytokine Netw. 24:11.
7. Mathieu ME, et al. 2012. Stem Cell Rev. 8:1.
8. Hu W, et al. 2007. Nature 450:721.
9. Trouillas M, et al. 2009. Eur. Cytokine Netw. 20:51.
10. Metcalfe SM. 2011. Genes Immun. 3:157.
Gene ID: 3976 View all products for this Gene ID
UniProt: View information about LIF on UniProt.org