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Purified anti-human CCL17 (TARC) Antibody
产品名称:
Purified anti-human CCL17 (TARC) Antibody
产品类别:
抗体
产品编号:
523002
产品应用:
523002
[价格]
规格 价格 库存
100µg ¥ 4900 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human CCL17
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.125-0.5 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of CCL17 (TARC) recombinant protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate.?For Western blotting, the suggested use of this reagent is 0.5 - 2.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified Poly5130 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the ?biotinylated Poly5230 antibody (Cat. No. 523003) as the detection antibody and recombinant human CCL17 (TARC) (Cat. No. 573809) as the standard.

Note: For testing human CCL17 (TARC) in serum, plasma or supernatant, BioLegend's ELISA LEGEND MAX? Kits (Cat. No. 441107 & 441108) are specially developed and recommended.

RRID
AB_2562135 (BioLegend Cat. No. 523002)

Antigen Details

Structure
Chemokine.
Distribution
Upon activation, CCL17 is expressed by PBMC, monocytes, dendritic cells, bronchial epithelial cells, smooth muscle cells, keratinocytes, and fibroblasts.
Function
CCL17 chemoattracts T cells, Th2 cells, and CLA+ CD4+ T cells and plays major role in allergic diseases. CCL17 can be upregulated by IL-4 in T cells and by a combination of cytokines in different cells.
Interaction
T cells, Th2 cells, CLA+ CD4 T cells, T (reg) cells, NK cells, NKT cells, and macrophages.
Ligand/Receptor
CCR4.
Cell Type
Monocytes, Dendritic cells, Epithelial cells, Fibroblasts
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Imai T, et al. 1997. J. Biol. Chem. 272:15036.
2. McIlroy A, et al. 2006. Immunology 117:507.
3. Yogo Y, et al. 2009. Respir. Res. 10:80.
4. Stutte S, et al. 2010. Proc. Natl. Acad. Sci. USA 107:8736.
5. Oo YH, et al. 2010. J. Immunol. 184:2886.

Gene ID
6361 View all products for this Gene ID
UniProt
View information about CCL17 on UniProt.org

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