Biotin anti-rat IFN-γ Antibody
- 产品名称:
- Biotin anti-rat IFN-γ Antibody
- 产品类别:
- 抗体
- 产品编号:
- 518803
- 产品应用:
- 518803
- Verified Reactivity
- Rat
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant rat IFN-γ
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.1 - 0.5 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of rat IFN-γ recombinant protein ranging from 2000 to 31 pg/mL are recommended for each ELISA plate. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection: The biotinylated poly5188 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified DB-1 antibody (Cat. No. 507801 or 507808) as the capture antibody and recombinant rat IFN-γ (Cat. No. 580009) as the standard.
Note: For testing rat IFN-γ in serum, plasma or cell culture supernatant, BioLegend's ELISA Legend Max? Kits (Cat. No. 439007 & 439008) are specially developed and recommended.
- Product Citations
-
- Allen KD, et al. 2020. Connect Tissue Res. 61:82. PubMed
- RRID
- AB_2561418 (BioLegend Cat. No. 518803)
- Structure
- Cytokine, dimer, 40-80 kD (Mammalian)
- Distribution
- CD8+ and CD4+ T cells, NK cells
- Function
- Upregulated by IL-2, bFGF, EGF, downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
- Interaction
- T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
- Ligand/Receptor
- IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
- Cell Type
- T cells, NK cells
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.
- Gene ID
- 25712 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org