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Biotin anti-rat IFN-γ Antibody
产品名称:
Biotin anti-rat IFN-γ Antibody
产品类别:
抗体
产品编号:
518803
产品应用:
518803
[价格]
规格 价格 库存
50µg ¥ 5250 1

产品详情

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant rat IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.1 - 0.5 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of rat IFN-γ recombinant protein ranging from 2000 to 31 pg/mL are recommended for each ELISA plate. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated poly5188 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified DB-1 antibody (Cat. No. 507801 or 507808) as the capture antibody and recombinant rat IFN-γ (Cat. No. 580009) as the standard.

Note: For testing rat IFN-γ in serum, plasma or cell culture supernatant, BioLegend's ELISA Legend Max? Kits (Cat. No. 439007 & 439008) are specially developed and recommended.

Product Citations
  1. Allen KD, et al. 2020. Connect Tissue Res. 61:82. PubMed
RRID
AB_2561418 (BioLegend Cat. No. 518803)

Antigen Details

Structure
Cytokine, dimer, 40-80 kD (Mammalian)
Distribution
CD8+ and CD4+ T cells, NK cells
Function
Upregulated by IL-2, bFGF, EGF, downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Interaction
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
T cells, NK cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Gene ID
25712 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

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