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Biotin anti-human IL-1β Antibody
产品名称:
Biotin anti-human IL-1β Antibody
产品类别:
抗体
产品编号:
517403
产品应用:
517403
[价格]
规格 价格 库存
50µg ¥ 5250 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human IL-1 beta
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-1β protein ranging from 1000 to 15.6 pg/ml are recommended for each ELISA plate when paired with H1b-27 (Cat. No. 511601). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated Poly5174 is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified H1b-27 antibody (Cat. No. 511601) as the capture antibody and recombinant human IL-1β (Cat. No. 579409) as the standard.

Note: For testing human IL-1β in serum or plasma, BioLegend's LEGEND MAX? Kits (Cat. No. 437007 & 437008) are specially developed and recommended.

RRID
AB_2561336 (BioLegend Cat. No. 517403)

Antigen Details

Structure
Cytokine, 17.3 kD.
Distribution

Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes.

Interaction
B cells, T cells, monocytes.
Ligand/Receptor
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b).
Cell Type
Astrocytes, B cells, Dendritic cells, Endothelial cells, Fibroblasts, Langerhans cells, Macrophages, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

Gene ID
3553 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org

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