| 规格 | 价格 | 库存 |
|---|---|---|
| 50µg | ¥ 5250 | 1 |
ELISA Detection - Quality tested
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-1β protein ranging from 1000 to 15.6 pg/ml are recommended for each ELISA plate when paired with H1b-27 (Cat. No. 511601). It is recommended that the reagent be titrated for optimal performance for each application.
ELISA Detection: The biotinylated Poly5174 is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified H1b-27 antibody (Cat. No. 511601) as the capture antibody and recombinant human IL-1β (Cat. No. 579409) as the standard.
Note: For testing human IL-1β in serum or plasma, BioLegend's LEGEND MAX? Kits (Cat. No. 437007 & 437008) are specially developed and recommended.
Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes.
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.