Purified Non-muscle Myosin Heavy Chain II-B Antibo
- 产品名称：
- Purified Non-muscle Myosin Heavy Chain II-B Antibo
- 产品类别：
- 抗体
- 产品编号：
- 909901
- 产品应用：
- 909901
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This antibody was raised against the peptide SDVNETQPPQSE corresponding to the C-terminus of nonmuscle myosin heavy chain isoform B (MHC-B).
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB - Quality tested
ICC - Verified
IHC-P, IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 ?g per ml (1:1000 - 1:2000).?For immunocytochemistry, the suggested use of this reagent is 0.5 - 2.0 ?g per ml (1:500 - 1:2000). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC) and immunofluorescence (IF).
*Predicted MW = 200 kD
Positive Control: human smooth muscle
This product may contain other non-IgG subtypes.
- Application References
(PubMed link indicates BioLegend citation) -
- Ma X, et al. 2007. Mol Biol Cell. 18:2305. (IF)
- Golomb E, et al. 2004. J Biol Chem. 279:2800. (WB, IF)
- Song J, et al. 2001. Mol Cell Neurosci. 17:624.
- Kelley CA, et al. 1996. J Cell Biol. 134:675.
- Kelley CA, et al. 1995. J Biol Chem. 270:1395.
- Phillips CL, et al. 1995. J Muscl Res Cell Motil. 16:379.
- Skau CT, et al. 2015. PNAS. 112:2447. PubMed
- Wang H, et al. 2008. J. Cell Biol. 182: 1171. (ICC, WB) PubMed
- Hirota Y, et al. 2010. Development. 137: 3037. (IF) PubMed
- Product Citations
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- RRID
- AB_2749903 (BioLegend Cat. No. 909902) AB_2565101 (BioLegend Cat. No. 909901)
