Purified Non-muscle Myosin Heavy Chain II-A Antibo
- 产品名称：
- Purified Non-muscle Myosin Heavy Chain II-A Antibo
- 产品类别：
- 抗体
- 产品编号：
- 909802
- 产品应用：
- 909802
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- Cow
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This antibody was raised against the peptide GKADGAEAKPAE corresponding to the C-terminus of human nonmuscle myosin heavy chain isoform A (MHC-A, NMHC IIA, Myosin-9).
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB -?Quality tested
ICC -?Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 ?g per ml (1:500 - 1:1000 dilution).?For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/ml (1:500 - 1:1000 dilution) is recommended. The recommended usage for this polyclonal antibody may vary from lot to lot. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody is effective in immunoblotting (WB) and immunofluorescence (IF).
*Predicted MW = 200 kD
This product may contain other non-IgG subtypes.
- Application References
(PubMed link indicates BioLegend citation) -
- Hirota Y, et al.. 2010. Development 137: 3037. (IF) PubMed
- Ma X, et al. 2007. Mol Biol Cell. 18:2305. (IF)
- Golomb E, et al. 2004. J Biol Chem. 279:2800. (WB, IF)
- Song J, et al. 2001. Mol Cell Neurosci. 17:624.
- Kelley CA, et al. 1996. J Cell Biol. 134:675.
- Kelley CA, et al. 1995. J Biol Chem. 270:1395.
- Wang H, et al. 2008. J. Cell Biol. 182: 1171. (ICC, WB) PubMed
- Product Citations
-
- Chopra A, et al. 2018. Dev Cell. 44:87. PubMed
- Schell C, et al. 2018. Dev Cell. 47:741. PubMed
- Kokate SB, et al. 2022. Nat Commun. 13:6032. PubMed
- Priya R, et al. 2017. Mol Biol Cell. 28(1):12-20. PubMed
- Gebala V, et al. 2016. Nat Cell Biol. 10.1038/ncb3320. PubMed
- Schiffhauer ES, et al. 2019. J Cell Biol. 218:895. PubMed
- Trela E, et al. 2021. J Cell Biol. 220:. PubMed
- Aguilar-Cuenca R, et al. 2020. Current Biology. 30(13):2446-2458.e6. PubMed
- Indra I, et al. 2020. Cell Rep. 30:2820. PubMed
- Chen P, et al. 2016. J Biol Chem. 291: 7517-7526. PubMed
- Otterpohl K, et al. 2017. Physiol Rep.. 10.14814/phy2.13513. PubMed
- Masedunskas A, et al. 2018. J Cell Sci. 131:6. PubMed
- Sumigray KD et al. 2018. Developmental cell. 45(2):183-197 . PubMed
- Meddens M, et al. 2016. Nat Commun. 7:13127. PubMed
- Lehtimki JI, et al. 2021. eLife. 10:00. PubMed
- Jiu Y, et al. 2019. Curr Biol. 29:81. PubMed
- Eaton AF, et al. 2019. Mol Biol Cell. 30:2037. PubMed
- Rajakyl ? EK, et al. 2020. Cell Rep. 30:4266. PubMed
- Weienbruch K, et al. 2021. eLife. 0.416666666666667. PubMed
- Salomon J, et al. 2017. Nat Commun. 8:13998. PubMed
- Hirota Y, et al. 2010. Development. 137:3037-3046. PubMed
- Cheng C, et al. 2018. J Cell Sci. 131. PubMed
- Lechuga S, et al. 2022. FASEB J. 36:e22290. PubMed
- Makowska K, et al. 2015. Cell Rep. 13: 2118-2125. PubMed
- Yu H, et al. 2016. J Cell Sci. 129: 80 - 94. PubMed
- Wang F, et al. 2018. J Cell Biol. 217:4314. PubMed
- Taneja N, et al. 2019. Mol Biol Cell. 30:1051. PubMed
- Pfisterer SG, et al. 2017. Nat Commun. 8:14858. PubMed
- Fukuda SP, et al. 2017. Dev Growth Differ. 59:423. PubMed
- Fredriksson-Lidman K, et al. 2017. PLoS One. 10.1371/journal.pone.0185509. PubMed
- Cechova S, et al. 2018. J Am Soc Nephrol. 29:155. PubMed
- Zhovmer AS, et al. 2019. Mol Biol Cell. 30:1961. PubMed
- Kumari S, et al. 2020. EMBO J. 39:e102783. PubMed
- Naydenov NG, et al. 2022. Cells. 11:. PubMed
- Miller MR, et al. 2021. Mol Biol Cell. :mbcE21050268. PubMed
- Taneja N, et al. 2020. Cell Rep. 31:107477. PubMed
- Hu F, et al. 2022. iScience. 25:105514. PubMed
- Murakami K, et al. 2016. Diabetes. 65: 1255 - 1267. PubMed
- Le H, et al. 2016. Nat Cell Biol. 18: 864-875. PubMed
- Choi J, et al. 2019. J Cell Biol. 218:2277. PubMed
- Chen B, et al. 2022. Nat Methods. 19:1419. PubMed
- Zaman R, et al. 2021. J Cell Biol. 220:. PubMed
- Gaston C, et al. 2021. Nat Commun. 12:2226. PubMed
- Wang H, et al. 2008. J Cell Biol. 182:1171-1184. PubMed
- Van Itallie CM, et al. 2018. J Cell Sci. 131:. PubMed
- Ciuba K, et al. 2018. Sci Rep. 8:17670. PubMed
- Ning W, et al. 2020. Cell Stem Cell. 28(3):436-452.e5. PubMed
- Nguyen-Ngoc K, et al. 2017. J Cell Sci. 10.1242/jcs.208546. PubMed
- RRID
- AB_2734686 (BioLegend Cat. No. 909802) AB_2565100 (BioLegend Cat. No. 909801)
