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Alexa Fluor® 488 anti-Giantin Antibody (Previo
产品名称:
Alexa Fluor® 488 anti-Giantin Antibody (Previo
产品类别:
抗体
产品编号:
908701
产品应用:
908701
[价格]
规格 价格 库存
100µL ¥ 4858 1

产品详情

Product Details

Verified Reactivity
Human, Monkey, Mouse, Rat, Canine
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This polyclonal antibody was raised against the N-terminus, residues 1-469, of human giantin.
Formulation
Phosphate-buffered solution + 50% glycerol + 2mM Sodium Azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
1 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution range of 1:250 - 1:1000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

This antibody is effective in immunoblotting (WB) and immunocytochemistry (ICC).

*Predicted MW = 367 kD

Western transfer should be conducted in the presence of 0.1% SDS. After transfer, rinse blot with 50% MeOH to remove any residual SDS.

This antibody is covalently coupled to Alexa Fluor? 488 to generate a one-step staining reagent; no secondary antibody detection is required. Alexa Fluor? 488 is an excellent alternative to fluorescein (FITC) dyes.

Application References

(PubMed link indicates BioLegend citation)
  1. Linstedt AD, Hauri HP. 1993. Mol Biol Cell. 4:679.
  2. Kong S, et al. 2013. Cell Res. 23:835. (IF) PubMed
Product Citations
  1. Cha JH et al. 2018. Molecular cell. 71(4):606-620 . PubMed
  2. Lam MT, et al. 2019. J Exp Med. 216:2778. PubMed
  3. Kong S, et al. 2013. Cell Res. 23:835-850. PubMed
  4. Cabanes-Creus M, et al. 2021. Mol Ther Methods Clin Dev. 21:607. PubMed
RRID
AB_2565089 (BioLegend Cat. No. 908701)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Golgi Markers
Gene ID
2804 View all products for this Gene ID
UniProt
View information about Giantin on UniProt.org

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