Alexa Fluor® 488 anti-Giantin Antibody (Previo
- 产品名称:
- Alexa Fluor® 488 anti-Giantin Antibody (Previo
- 产品类别:
- 抗体
- 产品编号:
- 908701
- 产品应用:
- 908701
- Verified Reactivity
- Human, Monkey, Mouse, Rat, Canine
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This polyclonal antibody was raised against the N-terminus, residues 1-469, of human giantin.
- Formulation
- Phosphate-buffered solution + 50% glycerol + 2mM Sodium Azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 1 mg/ml
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution range of 1:250 - 1:1000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
This antibody is effective in immunoblotting (WB) and immunocytochemistry (ICC).
*Predicted MW = 367 kD
Western transfer should be conducted in the presence of 0.1% SDS. After transfer, rinse blot with 50% MeOH to remove any residual SDS.
This antibody is covalently coupled to Alexa Fluor? 488 to generate a one-step staining reagent; no secondary antibody detection is required. Alexa Fluor? 488 is an excellent alternative to fluorescein (FITC) dyes.
- Application References
(PubMed link indicates BioLegend citation) -
- Linstedt AD, Hauri HP. 1993. Mol Biol Cell. 4:679.
- Kong S, et al. 2013. Cell Res. 23:835. (IF) PubMed
- Product Citations
-
- Cha JH et al. 2018. Molecular cell. 71(4):606-620 . PubMed
- Lam MT, et al. 2019. J Exp Med. 216:2778. PubMed
- Kong S, et al. 2013. Cell Res. 23:835-850. PubMed
- Cabanes-Creus M, et al. 2021. Mol Ther Methods Clin Dev. 21:607. PubMed
- RRID
- AB_2565089 (BioLegend Cat. No. 908701)