Purified anti-Keratin 1 Antibody (Previously Covan
- 产品名称：
- Purified anti-Keratin 1 Antibody (Previously Covan
- 产品类别：
- 抗体
- 产品编号：
- 905602
- 产品应用：
- 905602
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 1 protein.
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC-P - Quality tested
WB, ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution of 1:500 is suggested.?For Western blotting, the suggested use of this reagent is 1.0 ?g per mL?(1:1000 dilution).?For immunocytochemistry, a dilution of 1:500?is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody is effective in immunoblotting (WB), immunofluorescence (IF) and immunohistochemistry (IHC). This antibody has been successfully used in both frozen and paraffin embedded tissues.
*Predicted MW = 67 kD
This product may contain other non-IgG subtypes.
- Application References
(PubMed link indicates BioLegend citation) -
- Croyle MJ, et al. 2011. Development 138: 1675. (IHC, IF) PubMed
- Wirt SE, et al. 2010. J. Cell Biol. 191: 809. (IHC) PubMed
- Hu Y, et al. 2001. Nature. 410:710.
- Yuspa SH, et al. 1989. J Cell Biol. 109:1207.
- Roop DR, et al. 1984. J Biol Chem. 259:8037.
- Mailleux AA, et al. 2007. Dec Cell. 12:221. (IHC)
- IShitsuka Y, et al. 2013. J Invest Dermatol. 133:2566. (WB)
- Sengupta A, et al. 2010. PLoS One. 5:12249. (IHC) PubMed
- Suzuki K, et al. 2009. Development. 136:367. (IHC) PubMed
- Levy V, et al. 2007. FASEB J. 21:1358. (IHC) PubMed
- Osakarsson T, et al. 2006. Genes Dev. 20:2024. (IHC) PubMed
- Liang Y. 2011. Patholog Res Int. 2011:93674. (IHC) PubMed
- Sachs N, et al. 2012. PNAS. 109:21468. (IF)
- Vidal VP, et al. 2005. Curr Biol. 15:1340. (IHC)
- Product Citations
-
- Sengupta A, et al. 2010. PLoS One. 5:e12249. PubMed
- Suzuki K, et al. 2009. Development. 136:367-372. PubMed
- Kashgari G, et al. 2020. Developmental Cell. 52(6):764-778. PubMed
- Levy V, et al. 2007. FASEB J. 21:1358-1366. PubMed
- Ackermann T, et al. 2019. Commun Biol. 2:208. PubMed
- Linn SC, et al. 2019. Exp Dermatol. 28:383. PubMed
- Oskarsson T, et al. 2006. Genes Dev. 20:2024-2029. PubMed
- Tchegnon E, et al. 2021. JCI Insight. 6:. PubMed
- Tripurani SK, et al. 2018. Mol Biol Cell. 29:2784. PubMed
- Xu KK, et al. 2020. Invest Ophthalmol Vis Sci. 61:30:00. PubMed
- Wirt S, et al. 2010. J Cell Biol. 191:809-825. PubMed
- Weingarten G, et al. 2019. FASEB J. 33:2241. PubMed
- Liang Y 2011. Patholog Res Int. 2011:936794. PubMed
- Kok LF, et al. 2020. J Invest Dermatol. 140:1459. PubMed
- Li H, Zhou L, Dai J, et al. 2017. J Cell Physiol. 10.1002/jcp.25924. PubMed
- Yu Q, et al. 2021. eLife. 10:00. PubMed
- Brosseau JP, et al. 2018. Nat Commun. 9:5014. PubMed
- Croyle M, et al. 2011. Development. 138:1675-1685. PubMed
- Kang S, et al. 2020. Stem Cell Reports. 14:34. PubMed
- RRID
- AB_2801250 (BioLegend Cat. No. 905602) AB_2565051 (BioLegend Cat. No. 905601)
