Purified anti-Pax-6 Antibody (Previously Covance c-上海雅吉生物科技有限公司

规格	价格	库存
25µL	￥ 1904	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Polyclonal

Host Species

Rabbit

Immunogen

This antibody was generated against the peptide (QVPGSEPDMSQYWPRLQ) derived from the C-terminus of the mouse Pax-6 protein.

Formulation

Phosphate-buffered solution + 0.03% Thimerosal.

Preparation

The antibody was purified by affinity chromatography.

Concentration

2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.

Application

WB, IHC-P - Quality tested
IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting and?formalin-fixed paraffin-embedded immunohistochemical staining of brain tissue.?For Western blotting, the suggested use of this reagent is 1.0 ?g/mL?(1:2000).?For immunohistochemistry, a dilution of 1:50 - 1:100 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody weakly reacts with rat. It?has also shown reactivity with GFP and is therefore not recommended for use in GFP-expressing systems (as determined by in-house testing).

Pax-6 has two isoforms in human and mice at?46.6 and 48.2 Kd. This antibody recognizes both. The sequence is highly conserved among Pax-6 of various species. The antibody was subsequently purified on a Protein A column and is useful in studying brain, neuronal and olfactory development in higher eukaryotes.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References (PubMed link indicates BioLegend citation)

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