PerCP/Cyanine5.5 anti-mouse CD86 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD86 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 105127
- 产品应用：
- 105127
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- BALB/c mouse B leukemia cell line BCL1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking 1,4,5 of autoantibody production and T cell activation, stimulation of B cell activity3, immunoprecipitation2, and immunohistochemical staining2 of acetone-fixed frozen sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Nakajima A, et al. 1995. Eur. J. Immunol. 25:3060.
- Nuriya S, et al. 1996. Int. Immunol. 8:917.
- Kasprowicz DJ, et al. 2000. J. Immunol. 165:680.
- Saito K, et al. 1998. J. Immunol. 160:4225.
- Nakajima A, et al. 1998. J. Immunol. 161:1901.
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200.
- Giroux M, et al. 2007. J. Immunol. 179:4492.PubMed
- RRID
- AB_2924444 (BioLegend Cat. No. 105127) AB_2924444 (BioLegend Cat. No. 105128)
- Structure
- Ig superfamily, 80 kD
- Distribution
-
B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
- Function
- T cell costimulation, Ig class-switching, NK cell cytotoxicity
- Ligand/Receptor
- CD28, CD152 (CTLA-4)
- Cell Type
- Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science. 262:905.
3. Freeman GJ, et al. 1993. Science. 262:907.
4. Carreno BM, et al. 2002. Annu?Rev?Immunol. 20:29.
- Gene ID
- 12524 View all products for this Gene ID
- UniProt
- View information about CD86 on UniProt.org
