APC anti-mouse CD86 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1330	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

BALB/c mouse B leukemia cell line BCL1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking ^1,4,5 of autoantibody production and T cell activation, stimulation of B cell activity³, immunoprecipitation², and immunohistochemical staining² of acetone-fixed frozen sections.

Application References (PubMed link indicates BioLegend citation)

Nakajima A, et al. 1995. Eur. J. Immunol. 25:3060.
Nuriya S, et al. 1996. Int. Immunol. 8:917.
Kasprowicz DJ, et al. 2000. J. Immunol. 165:680.
Saito K, et al. 1998. J. Immunol. 160:4225.
Nakajima A, et al. 1998. J. Immunol. 161:1901.
Ma XT, et al. 2006. Cancer Research 66:1169.
Lawson BR, et al. 2007. J. Immunol. 178:5366.
Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200.
Giroux M, et al. 2007. J. Immunol. 179:4492.PubMed

Product Citations

Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
Ling X, et al. 2021. ACS Nano. 15:765. PubMed
Verteuil D, et al. 2010. Mol Cell Proteomics. 1.7875. PubMed
Taddio MF, et al. 2021. Mol Imaging Biol. 23:196. PubMed
Li J, et al. 2022. Nat Commun. 13:4032. PubMed
Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
Ling X, et al. 2021. Biomaterials. 270:120690. PubMed
Liu Y, et al. 2019. Am J Physiol Cell Physiol. 317:C762. PubMed

RRID

AB_313166 (BioLegend Cat. No. 105113) AB_313167 (BioLegend Cat. No. 105114)

Antigen Details

Structure: Ig superfamily, 80 kD
Distribution: B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
Function: T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science. 262:905.
3. Freeman GJ, et al. 1993. Science. 262:907.
4. Carreno BM, et al. 2002. Annu?Rev?Immunol. 20:29.
Gene ID: 12524 View all products for this Gene ID
UniProt: View information about CD86 on UniProt.org

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