Alexa Fluor® 700 anti-mouse CD86 Antibody
- 产品名称：
- Alexa Fluor® 700 anti-mouse CD86 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 105122
- 产品应用：
- 105122
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- BALB/c mouse B leukemia cell line BCL1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking 1,4,5 of autoantibody production and T cell activation, stimulation of B cell activity3, immunoprecipitation2, and immunohistochemical staining2 of acetone-fixed frozen sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Nakajima A, et al. 1995. Eur. J. Immunol. 25:3060.
- Nuriya S, et al. 1996. Int. Immunol. 8:917.
- Kasprowicz DJ, et al. 2000. J. Immunol. 165:680.
- Saito K, et al. 1998. J. Immunol. 160:4225.
- Nakajima A, et al. 1998. J. Immunol. 161:1901.
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200.
- Giroux M, et al. 2007. J. Immunol. 179:4492.PubMed
- Product Citations
-
- Luger R, et al. 2013. PLoS One. 8:e54879. PubMed
- Boldison J, et al. 2020. Cell Mol Immunol. 17:843. PubMed
- Mayer E, et al. 2013. Int Immunopharmacol. 15:638. PubMed
- RRID
- AB_493723 (BioLegend Cat. No. 105122)
- Structure
- Ig superfamily, 80 kD
- Distribution
-
B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
- Function
- T cell costimulation, Ig class-switching, NK cell cytotoxicity
- Ligand/Receptor
- CD28, CD152 (CTLA-4)
- Cell Type
- Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science. 262:905.
3. Freeman GJ, et al. 1993. Science. 262:907.
4. Carreno BM, et al. 2002. Annu?Rev?Immunol. 20:29.
- Gene ID
- 12524 View all products for this Gene ID
- UniProt
- View information about CD86 on UniProt.org
