Alexa Fluor® 488 anti-mouse Podoplanin Antibod
- 产品名称：
- Alexa Fluor® 488 anti-mouse Podoplanin Antibod
- 产品类别：
- 抗体
- 产品编号：
- 156207
- 产品应用：
- 156207
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Peptide (38-51 amino acids of mouse podoplanin)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IHC-F, 3D IHC -?Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. For immunohistochemistry, a concentration range of 5.0?- 10??g/ml is suggested.?For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Yamada S, et al. 2017. Monoclon Antib Immunodiagn Immunother. 36(2):77-79.
- Product Citations
-
- Mece O, et al. 2022. Nat Commun. 13:2760. PubMed
- RRID
- AB_2814079 (BioLegend Cat. No. 156207) AB_2814080 (BioLegend Cat. No. 156208)
- Structure
- 43 kD glycosylated type-1 transmembrane protein. Mucin-type protein.
- Distribution
-
Expressed on epithelial and mesothelial cells.
- Function
- Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation.
- Interaction
- Interacts with CLEC1B; the interaction is independent of CLEC1B glycosylation and activates CLEC1B; the interaction is dependent of sialic acid on O-glycans.
- Ligand/Receptor
- CLEC1B
- Cell Type
- Epithelial cells
- Biology Area
- Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
- Farr A, et al. 1992. J Histochem Cytochem. 40:651.
- Schacht V, et al. 2005. Am J Pathol. 166:913.
- Gene ID
- 14726 View all products for this Gene ID
- UniProt
- View information about Podoplanin on UniProt.org
