Purified anti-mouse NK-1.1 (Maxpar® Ready) Ant-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1484	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

NK-1⁺ cells from mouse spleen and bone marrow

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, complement-dependent cytotoxicity³, in vivo depletion^4,5,9,10, mediation of in vitro redirected lysis⁶, blocking of NK cell function⁷, induction of proliferation⁸, immunohistochemical staining of frozen sections¹¹, immunofluorescence microscopy¹¹, and spatial biology (IBEX)^16,17. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 108712).

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
Wei SC, et al. 2019. Immunity. 50:1084. PubMed
McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed
Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed

RRID

AB_2562803 (BioLegend Cat. No. 108743)

Antigen Details

Structure: NKR-P1 gene family
Distribution: NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)
Function: NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.
Gene ID: 17059 View all products for this Gene ID
UniProt: View information about NK-1.1 on UniProt.org

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