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PerCP/Cyanine5.5 anti-mouse NK-1.1 Antibody
产品名称：
PerCP/Cyanine5.5 anti-mouse NK-1.1 Antibody
产品类别：
抗体
产品编号：
108727
产品应用：
108727
[价格]
规格 价格 库存
25µg ￥ 1302 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 108712).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  17. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Minute L, et al. 2020. J Immunother Cancer. 8:. PubMed
  2. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  3. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  4. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  5. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  6. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  7. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  8. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  9. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  10. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  11. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  12. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  13. Park D, et al. 2020. Cancer Res. 80:4172. PubMed
  14. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  15. Abdul Pari AA, et al. 2020. Cancer Res. 80:2586. PubMed
  16. Kooreman NG et al. 2018. Cell stem cell. 22(4):501-513 . PubMed
  17. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  18. Peauroi EM, et al. 2022. iScience. 25:105510. PubMed
  19. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  20. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  21. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  22. Liyanage S, et al. 2016. Exp Eye Res. 151:160-70. PubMed
  23. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  24. Monaghan KL, et al. 2020. J Vis Exp. . PubMed
  25. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  26. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  27. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  28. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  29. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  30. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  31. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  32. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  33. Rosato PC, et al. 2019. Nat Commun. 10:567. PubMed
  34. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  35. Gardner P, et al. 2017. Sci Rep. 7:40830. PubMed
  36. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  37. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  38. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  39. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  40. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  41. Gawish R, et al. 2022. Elife. 11:. PubMed
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  43. Tian L, et al. 2012. J Autoimmun. 38:39. PubMed
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RRID
AB_2132706 (BioLegend Cat. No. 108727) AB_2132705 (BioLegend Cat. No. 108728)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

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